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ZFIN ID: ZDB-FIG-110408-3
Corti et al., 2011 - Interaction between alk1 and blood flow in the development of arteriovenous malformations. Development (Cambridge, England)   138(8):1573-1582 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25

Fig. 3

alk1 expression requires blood flow. (A) Spatiotemporal pattern of alk1 mRNA expression assayed by whole-mount in situ hybridization. Expression is detected in the first aortic arch (AA1, white asterisk), lateral dorsal aorta (LDA; blue arrowhead) and internal carotid artery (ICA, blue arrow) at 26-28 hpf, then in the caudal division of the internal carotid artery (CaDI, white arrow) and basal communicating artery (BCA, white arrowhead) by 28-30 hpf. Tracings in the far right column represent all vessels expressing cdh5 at 36 hpf, with alk1-positive arteries in pink, alk1-negative arteries in red and veins in black or gray. BA, basilar artery; LDA, lateral dorsal aortae; MtA, metencephalic artery; OA, optic artery; PCS, posterior communicating segments. Lateral and dorsal views, anterior leftwards. Frontal view, anterior rightwards. Scale bar: 50 μm. (B) Onset of blood flow correlates with alk1 expression. At 26 hpf, blood flows caudally through AA1 (asterisk) and the LDA (blue arrowhead). The cranialward ICA (blue arrow) and CaDI (white arrow) carry flow by 28 hpf; the BCA (white arrowhead) by 30 hpf; and the PCSs (blue asterisks) by 32 hpf. Images are two-dimensional confocal projections of chrna1-/- (paralyzed), Tg(kdrl:GFP)la116;Tg(gata1:dsRed)sd2 embryos. Endothelial cells are green, erythrocytes are magenta. Lateral and dorsal views (row 2, columns 3-5), anterior leftwards. Frontal view (row 2, columns 1-2), anterior rightwards. Scale bar: 50 μm. (C) Whole-mount in situ hybridization demonstrates that alk1 is downregulated in the absence of blood flow (tnnt2a-/- or tricaine treatment, 32-40 hpf) but is not affected by the absence of erythrocytes (gata1a-/-). cdh5 expression is unchanged under all conditions. All embryos are at 36 hpf except tricaine treated, which are at 40 hpf. Lateral views, anterior leftwards. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
acvrl1 WT standard conditions Prim-5 aortic arch 1 ISH
Prim-5 internal carotid artery ISH
Prim-5 lateral dorsal aorta ISH
Prim-15 aortic arch 1 ISH
Prim-15 caudal division of the internal carotid artery ISH
Prim-15 internal carotid artery ISH
Prim-15 lateral dorsal aorta ISH
Prim-25 aortic arch 1 ISH
Prim-25 basal communicating artery ISH
Prim-25 caudal division of the internal carotid artery ISH
Prim-25 internal carotid artery ISH
Prim-25 lateral dorsal aorta ISH
WT chemical treatment: pharmaceutical Prim-25 Not Detected blood vessel ISH
gata1am651/m651 standard conditions Prim-25 blood vessel ISH
tnnt2atc300b/tc300b standard conditions Prim-25 Not Detected blood vessel ISH
cdh5 WT standard conditions Prim-25 blood vessel ISH
WT chemical treatment: pharmaceutical Prim-25 blood vessel ISH
gata1am651/m651 standard conditions Prim-25 blood vessel ISH
tnnt2atc300b/tc300b standard conditions Prim-25 blood vessel ISH
DsRed chrna1b107/b107; la116Tg/la116Tg; sd2Tg/sd2Tg standard conditions Prim-5 nucleate erythrocyte IFL
Prim-15 nucleate erythrocyte IFL
EGFP chrna1b107/b107; la116Tg/la116Tg; sd2Tg/sd2Tg standard conditions Prim-5 endothelial cell IFL
Prim-15 endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development