ZFIN ID: ZDB-FIG-110221-13
Li et al., 2011 - Rack1 is required for Vangl2 membrane localization and planar cell polarity signaling while attenuating canonical Wnt activity. Proceedings of the National Academy of Sciences of the United States of America   108(6):2264-2269 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 30%-epiboly to 75%-epiboly

Fig. 4 Rack1 is required for CE during gastrulation. (A) The diagrams illustrate the directions of cellular movement during zebrafish gastrulation. (B) Images of live embryos injected with vangl2 MO, rack1 MO, or rack1 MO plus mouse rack1 mRNA and subsequently labeled single blastomere cell with fluorescein dextran at the 32-cell stage. V, ventral; D, dorsal.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-vangl2 standard conditions 30%-epiboly convergent extension involved in gastrulation disrupted, abnormal
Shield convergent extension involved in gastrulation disrupted, abnormal
75%-epiboly convergent extension involved in gastrulation disrupted, abnormal
WT + MO3-rack1 standard conditions 30%-epiboly convergent extension involved in gastrulation disrupted, abnormal
Shield convergent extension involved in gastrulation disrupted, abnormal
75%-epiboly convergent extension involved in gastrulation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA