FIGURE

Fig. 2

ID

ZDB-FIG-110221-11

Publication

Li et al., 2011 - Rack1 is required for Vangl2 membrane localization and planar cell polarity signaling while attenuating canonical Wnt activity

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Fig. 2

Rack1 is required for zebrafish gastrulation. (A–C) Both rack1 and vangl2 morphants exhibited a shortened anterior–posterior axis as well as widened somites as shown by lateral (A) and dorsal (B) views of live embryos (B Upper) and embryos hybridized with myoD1 in situ probes (B Lower). The images in A and B are representative of each group of ∼200 embryos (C). Note that cellular shedding at the dorsal side was observed in rack1 or rack1 and vangl2 morphants but not vangl2 morphants. (D) Although Rack1 protein is abundant in control embryos of six-somite stage, it is barely detectable in rack1 morphant embryos of the same stage. a, anterior; p, posterior; n, notochord; s, somite.

Expression Data

Gene:	rack1
Fish:	WT WT + MO3-rack1
Knockdown Reagent:	MO3-rack1
Anatomical Term:	whole organism
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-vangl2 WT + MO3-rack1 WT + MO1-vangl2 + MO3-rack1
Knockdown Reagents:	MO1-vangl2 MO3-rack1
Observed In:	notochord somite whole organism
Stage Range:	5-9 somites to 10-13 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA