ZFIN ID: ZDB-FIG-110221-11
Li et al., 2011 - Rack1 is required for Vangl2 membrane localization and planar cell polarity signaling while attenuating canonical Wnt activity. Proceedings of the National Academy of Sciences of the United States of America   108(6):2264-2269 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: 5-9 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 5-9 somites to 10-13 somites

Fig. 2 Rack1 is required for zebrafish gastrulation. (A–C) Both rack1 and vangl2 morphants exhibited a shortened anterior–posterior axis as well as widened somites as shown by lateral (A) and dorsal (B) views of live embryos (B Upper) and embryos hybridized with myoD1 in situ probes (B Lower). The images in A and B are representative of each group of ∼200 embryos (C). Note that cellular shedding at the dorsal side was observed in rack1 or rack1 and vangl2 morphants but not vangl2 morphants. (D) Although Rack1 protein is abundant in control embryos of six-somite stage, it is barely detectable in rack1 morphant embryos of the same stage. a, anterior; p, posterior; n, notochord; s, somite.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
rack1 WT control 5-9 somites whole organism WB
WT + MO3-rack1 standard conditions 5-9 somites Not Detected whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-vangl2 standard conditions 5-9 somites - 10-13 somites notochord undulate, abnormal
5-9 somites - 10-13 somites somite increased width, abnormal
5-9 somites - 10-13 somites whole organism decreased length, abnormal
WT + MO1-vangl2 + MO3-rack1 standard conditions 5-9 somites - 10-13 somites notochord undulate, abnormal
5-9 somites - 10-13 somites somite increased width, abnormal
5-9 somites - 10-13 somites whole organism decreased length, abnormal
WT + MO3-rack1 standard conditions 5-9 somites - 10-13 somites notochord undulate, abnormal
5-9 somites - 10-13 somites somite increased width, abnormal
5-9 somites - 10-13 somites whole organism decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA