|ZFIN ID: ZDB-FIG-110221-11|
|Stage Range:||5-9 somites to 10-13 somites|
Fig. 2 Rack1 is required for zebrafish gastrulation. (A–C) Both rack1 and vangl2 morphants exhibited a shortened anterior–posterior axis as well as widened somites as shown by lateral (A) and dorsal (B) views of live embryos (B Upper) and embryos hybridized with myoD1 in situ probes (B Lower). The images in A and B are representative of each group of ∼200 embryos (C). Note that cellular shedding at the dorsal side was observed in rack1 or rack1 and vangl2 morphants but not vangl2 morphants. (D) Although Rack1 protein is abundant in control embryos of six-somite stage, it is barely detectable in rack1 morphant embryos of the same stage. a, anterior; p, posterior; n, notochord; s, somite.
|rack1||WT||control||5-9 somites||whole organism||WB|
|WT + MO3-rack1||standard conditions||5-9 somites||Not Detected||whole organism||WB|
|Acknowledgments:||ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA|