FIGURE

Fig. S4

ID

ZDB-FIG-110221-17

Publication

Li et al., 2011 - Rack1 is required for Vangl2 membrane localization and planar cell polarity signaling while attenuating canonical Wnt activity

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Fig. S4

Rack1 is associated with Vangl2 in zebrafish embryo extracts. RNA encoding EGFP-Vangl2 (200 pg) was injected into zebrafish embryos at the one- or two-cell stage. The embryos were collected from the bud stage (10 hpf) to 10-somite stage (14 hpf) and processed for yolk removal and protein extraction from the dissected EGFP-Vangl2–expressing embryos. Similarly, extracts were made from control embryos. (A) Coimmunoprecipitation experiments were performed as described. Note that EGFP-Vangl2 was pulled down only in the presence of Rack1 antibody and that Rack1 was coimmunoprecipitated only in the presence of EGFP-Vangl2 and EGFP antibody. (B) The protein extracts from the control and EGFP-Vangl2–injected embryos were loaded directly and blotted with an antibody against Rack1 as input controls.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA