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ZFIN ID: ZDB-FIG-110221-12
Li et al., 2011 - Rack1 is required for Vangl2 membrane localization and planar cell polarity signaling while attenuating canonical Wnt activity. Proceedings of the National Academy of Sciences of the United States of America   108(6):2264-2269 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Shield to Bud

Fig. 3 Cellular polarization and oriented cell division are disrupted in rack1 morphants. (A and B) Cellular morphology in the notochord at the bud stage was visualized with fluorescein-labeled BODIPY ceramide (A Upper) and diagramed (A Lower). The angles formed between the long axis of each cell and the mediolateral axis of each embryo were calculated and plotted with the Oriana 3 program (B). (C and D) Embryos injected with control or rack1 MOs, or RNAs encoding Xdsh-D2, Histone 2B-RFP, and membrane GFP were imaged at the shield stage from the dorsal side to visualize the orientation of mitotic divisions in dorsal epiblast cells (C). The angles formed between the direction of each mitotic spindle and the animal–vegetal axis of each embryo were quantified (D). The yellow lines depict the orientation of mitotic spindles. A-V, animal–vegetal axis.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-vangl2 standard conditions Bud establishment of cell polarity disrupted, abnormal
Bud notochord increased width, abnormal
Bud notochord undulate, abnormal
WT + MO3-rack1 standard conditions Shield establishment of mitotic spindle orientation disrupted, abnormal
Bud establishment of cell polarity disrupted, abnormal
Bud notochord increased width, abnormal
Bud notochord undulate, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA