PUBLICATION

Rack1 is required for Vangl2 membrane localization and planar cell polarity signaling while attenuating canonical Wnt activity

Authors
Li, S., Esterberg, R., Lachance, V., Ren, D., Radde-Gallwitz, K., Chi, F., Parent, J.L., Fritz, A., and Chen, P.
ID
ZDB-PUB-110131-13
Date
2011
Source
Proceedings of the National Academy of Sciences of the United States of America   108(6): 2264-2269 (Journal)
Registered Authors
Fritz, Andreas
Keywords
none
MeSH Terms
  • Animals
  • Cell Division/physiology
  • Cell Membrane/genetics
  • Cell Membrane/metabolism
  • Cell Polarity/physiology*
  • Gastrula/cytology
  • Gastrula/metabolism*
  • Gastrulation/physiology*
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism*
  • Mice
  • Protein Structure, Tertiary
  • Protein Transport/physiology
  • Receptors, Cell Surface/genetics
  • Receptors, Cell Surface/metabolism*
  • Signal Transduction/physiology*
  • Wnt Proteins/genetics
  • Wnt Proteins/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
21262816 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
The vertebrate planar cell polarity (PCP) pathway shares molecular components with the β-catenin-mediated canonical Wnt pathway but acts through membrane complexes containing Vang or Frizzled to orient neighboring cells coordinately. The molecular interactions underlying the action of Vang in PCP signaling and specification, however, are yet to be delineated. Here, we report the identification of Rack1 as an interacting protein of a vertebrate Vang protein, Vangl2. We demonstrate that Rack1 is required in zebrafish for PCP-regulated processes, including oriented cell division, cellular polarization, and convergent extension during gastrulation. We further show that the knockdown of Rack1 affects membrane localization of Vangl2 and that the Vangl2-interacting domain of Rack1 has a dominant-negative effect on Vangl2 localization and gastrulation. Moreover, Rack1 antagonizes canonical Wnt signaling. Together, our data suggest that Rack1 regulates the localization of an essential PCP protein and acts as a molecular switch to promote PCP signaling.
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