FIGURE

Fig. 2

ID

ZDB-FIG-101223-4

Publication

Jackman et al., 2010 - Hedgehog signaling is required at multiple stages of zebrafish tooth development

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Fig. 2

Zebrafish mutant for shha do not exhibit tooth-related developmental gene expression and do not form mature teeth. (A, B) Ventral views of the pharyngeal region at 100 hpf stained with alizarin red to highlight calcified structures. Teeth appeared normal in wild-type and shha^t4heterozygous individuals (A), but were not detected in shha^t4homozygous mutant siblings (B). mRNA in situ hybridization analysis at 56 hpf demonstrated that pharyngeal tooth-related expression of dlx2b (C, D), fgf4 (E, F), lhx8a (G, H), and pitx2 (I, J) was completely absent from homozygous mutant embryos but present in sibling controls possessing at least one wild type shha allele. Expression of ptc1 in the tooth-forming region appeared to be maintained in all genotypes (K, L). (C-L) Dorsal views, anterior to the left, position of right side tooth germs indicated (arrows). Scale bars: (B) 50 μm, (D) 100 μm.

Expression Data

Genes:	dlx2b fgf4 lhx8a pitx2 ptch2
Fish:	Df(Chr07)t4/t4 WT
Anatomical Terms:	dental epithelium dental mesenchyme pharyngeal epithelium pharynx tooth placode
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	Df(Chr07)t4/t4
Observed In:	ceratobranchial 5 tooth odontogenesis whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.