ZFIN ID: ZDB-FIG-101223-4
Jackman et al., 2010 - Hedgehog signaling is required at multiple stages of zebrafish tooth development. BMC Developmental Biology   10:119 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 2 Zebrafish mutant for shha do not exhibit tooth-related developmental gene expression and do not form mature teeth. (A, B) Ventral views of the pharyngeal region at 100 hpf stained with alizarin red to highlight calcified structures. Teeth appeared normal in wild-type and shhat4 heterozygous individuals (A), but were not detected in shhat4 homozygous mutant siblings (B). mRNA in situ hybridization analysis at 56 hpf demonstrated that pharyngeal tooth-related expression of dlx2b (C, D), fgf4 (E, F), lhx8a (G, H), and pitx2 (I, J) was completely absent from homozygous mutant embryos but present in sibling controls possessing at least one wild type shha allele. Expression of ptc1 in the tooth-forming region appeared to be maintained in all genotypes (K, L). (C-L) Dorsal views, anterior to the left, position of right side tooth germs indicated (arrows). Scale bars: (B) 50 μm, (D) 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx2b WT standard conditions Long-pec tooth placode ISH
shhaDf(Chr07)t4/t4 standard conditions Long-pec Not Detected tooth placode ISH
fgf4 WT standard conditions Long-pec dental epithelium ISH
shhaDf(Chr07)t4/t4 standard conditions Long-pec Not Detected dental epithelium ISH
lhx8a WT standard conditions Long-pec dental mesenchyme ISH
shhaDf(Chr07)t4/t4 standard conditions Long-pec Not Detected dental mesenchyme ISH
pitx2 WT standard conditions Long-pec pharyngeal epithelium ISH
Long-pec tooth placode ISH
shhaDf(Chr07)t4/t4 standard conditions Long-pec Not Detected pharyngeal epithelium ISH
Long-pec Not Detected tooth placode ISH
ptch2 WT standard conditions Long-pec pharynx ISH
shhaDf(Chr07)t4/t4 standard conditions Long-pec pharynx ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
shhaDf(Chr07)t4/t4 standard conditions Day 4 odontogenesis arrested, abnormal
Day 4 whole organism lacks all parts of type ceratobranchial 5 tooth, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.