ZFIN ID: ZDB-FIG-101223-5
Jackman et al., 2010 - Hedgehog signaling is required at multiple stages of zebrafish tooth development. BMC Developmental Biology   10:119 Full text @ BMC Dev. Biol.
Knockdown Reagents:
Observed In:
Stage: Day 4

Fig. 3 Antisense RNA inhibition of shha, but not shhb, prevents the formation of mature teeth. Ventral views of the tooth-forming region of alizarin red stained 100 hpf embryos. Teeth develop normally in control morpholino injected embryos (A, left-side tooth shown in inset). Teeth are eliminated from shha MO injected embryos (B), but retained in embryos injected with an identical amount (12 ng) of the shhb morpholino (C). The combination of 6 ng shha MO with 18 ng shhb MO results in teeth forming closer to the midline and appearing slightly developmentally delayed but otherwise with normal morphology (D). Scale bar 100 μm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-shha standard conditions Day 4 odontogenesis arrested, abnormal
Day 4 whole organism lacks all parts of type ceratobranchial 5 tooth, abnormal
WT + MO1-shha + MO2-shhb standard conditions Day 4 odontogenesis delayed, abnormal
Day 4 tooth placode position, abnormal
WT + MO2-shhb standard conditions Day 4 odontogenesis arrested, abnormal
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.