FIGURE

Fig. 5

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ZDB-FIG-101011-50

Publication

Rai et al., 2010 - DNA demethylase activity maintains intestinal cells in an undifferentiated state following loss of APC

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Fig. 5

Lef1 and Groucho2 Suppress rdh1l in apc^mcr Zebrafish

(A) Whole-mount in situ staining for lef1, groucho2, tcf4, or groucho3 in apc^wt and apc^mcr embryos at 72 hpf. Red arrow points to the intestine.

(B) Whole-mount in situ staining for fabp2, trypsin, rdh1l, and irbp in apc^mcr/control Mo, apc^mcr /lef1 Mo, and apc^mcr /groucho2 Mo embryos at 72 hpf. Groucho2 mo efficiency is in Figure S5G.

(C and D) ChIP for Lef1 on rdh1l promoter in apc^wt and apc^mcr embryos.

(C) Schematic showing the Lef1 sites on the rdh1l promoter and location of the primer set P1 (containing Lef1 sites) and P2 (without Lef1 sites).

(D) Graph showing fold enrichment of Lef1 occupancy at P1/P2 on rdh1l promoter in apc^mcr embryos compared to apc^wt at 72 hpf. Lef1 values were normalized to ones obtained with a nonspecific antibody and then expressed as fold enrichment compared to apc^wt. Error bars indicate ± SD.

(E) Whole-mount in situ staining for fabp2 and gata6 in 72 hpf embryos injected with full-length lef1 or dominant negative (deficient in β-catenin binding) lef1 mRNA alone or with groucho2 mRNA.

See also Figure S5.

Expression Data

Genes:	dhrs9 fabp2 gata6 lef1 prss1 rbp3 tcf4 tle3a tle3b
Fish:	WT apc^hu745/hu745 apc^hu745/hu745 + MO1-tle3a apc^hu745/hu745 + MO2-lef1
Knockdown Reagents:	MO1-tle3a MO2-lef1
Anatomical Terms:	intestine pancreas retina
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	apc^hu745/hu745 apc^hu745/hu745 + MO1-tle3a apc^hu745/hu745 + MO2-lef1
Knockdown Reagents:	MO1-tle3a MO2-lef1
Observed In:	digestive tract mesoderm development intestine retina retina development in camera-type eye
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 142(6), Rai, K., Sarkar, S., Broadbent, T.J., Voas, M., Grossmann, K.F., Nadauld, L.D., Dehghanizadeh, S., Hagos, F.T., Li, Y., Toth, R.K., Chidester, S., Bahr, T.M., Johnson, W.E., Sklow, B., Burt, R., Cairns, B.R., and Jones, D.A., DNA demethylase activity maintains intestinal cells in an undifferentiated state following loss of APC, 930-942, Copyright (2010) with permission from Elsevier. Full text @ Cell