FIGURE

Fig. 2

ID

ZDB-FIG-100504-55

Publication

Hartnett et al., 2010 - Insulin-like growth factor-2 regulates early neural and cardiovascular system development in zebrafish embryos

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Fig. 2

IGF-2 is required for the development of anterior neural structures during gastrulation and plays an anti-apoptotic role during segmentation. (A,C) Control morpholino injected embryos show normal pax6.2 and rx3 expression. (B,D) IGF-2(a+b) morphant embryos show reductions in pax6.2 and rx3 expression. (E) Embryos injected with control morpholinos show low levels of apoptosis during zebrafish segmentation. (F) Knockdown of igf-2a and igf-2b results in an increase in apoptosis in the anterior region of the embryo and in the developing spinal cord. Frequency of embryos displaying this staining pattern; B, 14/ 32; D, 22/40; F, 17/28. (A-D) are views of the future anterior region; (E,F) are lateral views.

Expression Data

Genes:	pax6b rx3
Fish:	AB AB + MO1-igf2a + MO3-igf2b
Knockdown Reagents:	MO1-igf2a MO3-igf2b
Anatomical Terms:	axis forebrain neural keel optic primordium
Stage:	Bud

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-igf2a + MO3-igf2b
Knockdown Reagents:	MO1-igf2a MO3-igf2b
Observed In:	apoptotic process neural keel spinal cord neural keel
Stage:	Bud

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Dev. Biol.