FIGURE

Fig. 1

ID

ZDB-FIG-100504-54

Publication

Hartnett et al., 2010 - Insulin-like growth factor-2 regulates early neural and cardiovascular system development in zebrafish embryos

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Fig. 1

IGF-2 morpholinos efficiently and specifically knockdown IGF-2 in vivo. (A) Phenotype of (a) control injected and (b) IGF-2(a+b) morphant embryo at 24 hpf. IGF-2(a+b) morphant embryos are ventralised with a shorter body, disrupted brain structures (double arrows), reduced eyes, disrupted somites and an expanded intermediate cell mass (arrow). (B) Western blot showing IGF-2 and alpha-tubulin at 24 hpf in (1) uninjected control embryos, (2) embryos injected with control morpholinos or (3) embryos injected with IGF-2(a+b) morpholinos. (C) Igf-2a and igf-2b mediate their effects on development in a synergistic fashion. (D) Co-injection of igf-2a or igf-2b RNA with the corresponding morpholino rescues the IGF-2 morphant phenotype. Rescued embryos display normal body size, eyes and brain structures at 24 hpf. Results shown are a summary of three independent experiments (n ≥ 80). Error bars indicate the standard deviation of the mean.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-igf2a AB + MO3-igf2b AB + MO1-igf2a + MO3-igf2b
Knockdown Reagents:	MO1-igf2a MO3-igf2b
Observed In:	brain eye intermediate cell mass of mesoderm somite whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Dev. Biol.