FIGURE

Fig. 6

ID

ZDB-FIG-100304-6

Publication

Chen et al., 2010 - Nestin Is Essential for Zebrafish Brain and Eye Development through Control of Progenitor Cell Apoptosis

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Fig. 6

Effects of nestin MO injection on isl1, acetylated tubulin (AcTub) and gfap:GFP expression.

A. Isl1 expression was analyzed by WMISH at 25 hpf. Arrow indicates for brain and arrow head, spinal cord. B. Transgenic Tg(isl1:GFP) embryos were injected with nestin MO. GFP expression was examined under confocal microscopy at 49 hfp. Uninjected WT (no inject) was used as a control. C. AcTub expression (axon marker) was examined by confocal microscopy at 49 hpf. The upper panels show lateral view and the lower panels, dorsal view. D. Heterozygous Tg(gfap:GFP) embryos were injected with nestin MO and GFP was visualized under fluorescent microscopy at 49 hpf. Scale bars: 200 μm.

Expression Data

Genes:	GFP isl1a
Fish:	mi2001Tg/+ (AB) AB AB + MO1-nes mi2001Tg/+ + MO1-nes (AB)
Knockdown Reagent:	MO1-nes
Anatomical Terms:	brain glial cell spinal cord
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mi2001Tg/+ + MO1-nes (AB) rw0Tg + MO1-nes
Knockdown Reagent:	MO1-nes
Observed In:	cranial nerve III cranial nerve IV cranial nerve V cranial nerve VII cranial nerve X glial cell nervous system axon
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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