Fig. 2

Embryo morphological changes induced by nestin MO.

A–D. One-cell stage embryos were injected with 10 ng nestin MO and morphology was examined at 24 hpf and 28 hpf. Compared to normal morphology of wild-type (WT) (A: lateral view and C: dorsal view), nestin morphant exhibited head malformation (B: lateral view) with unclear boundaries between brain subdivisions, especially the hindbrain and midbrain boundary (D: dorsal view). E-J. Embryos were examined at 51 hpf after injection of 10 ng nestin MO1 or an equal amount of control MO and 5-mis nestin MO. The head abnormality had become more pronounced in nestin morphants with overt hydrocephalus (G and H). G illustrates mild and H, severe hydrocephalus. The morphology of control MO and 5-mis nestin MO remained normal (E and F). Dorsal view of nestin morphants revealed reduced size of tectum (J) compared with control morphants (I). K–N. Magnified views of E–H to illustrate the gross head and eye defects and hydrocephalus in nestin morphants. Blue circles in I and J refer to tectum. Blue double-head arrows in K–N denote diameter of the eyes. * denotes mild and ** severe hydrocephalus. The scale bar is 200 μm.