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Fig. 6 Effects of nestin MO injection on isl1, acetylated tubulin (AcTub) and gfap:GFP expression.
A. Isl1 expression was analyzed by WMISH at 25 hpf. Arrow indicates for brain and arrow head, spinal cord. B. Transgenic Tg(isl1:GFP) embryos were injected with nestin MO. GFP expression was examined under confocal microscopy at 49 hfp. Uninjected WT (no inject) was used as a control. C. AcTub expression (axon marker) was examined by confocal microscopy at 49 hpf. The upper panels show lateral view and the lower panels, dorsal view. D. Heterozygous Tg(gfap:GFP) embryos were injected with nestin MO and GFP was visualized under fluorescent microscopy at 49 hpf. Scale bars: 200 μm.