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ZFIN ID: ZDB-FIG-091217-76
Duldulao et al., 2009 - Cilia localization is essential for in vivo functions of the Joubert syndrome protein Arl13b/Scorpion. Development (Cambridge, England)   136(23):4033-4042 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 20-25 somites to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-15 to Day 5

Fig. 4 Cilia in scohi459 mutant embryos. (A,B) Confocal projections showing the pronephric duct in a wild-type embryo (A) and an arl13b morphant (B) at the 20-somite stage, stained with anti-acetylated tubulin (red) and anti-Sco (green). Signal in B is overexposed. Scale bars: 5 μm. (C,D) Confocal projections showing cilia defect (stained with anti-acetylated tubulin, red) in the pronephric duct (stained with anti-Cdh17, green) in a scohi459 mutant embryo (D), compared with a wild-type sibling (C), at 33 hpf. Scale bars: 10 μm. (E,F) Confocal projection showing cilia (stained with anti-acetylated tubulin, red) in the pronephric duct (stained with anti-Cdh17, green) in embryos at 5 dpf. Scale bar: 20 μm. (G,H) Electron micrographs showing the ultrastructure of cilia in the scohi459 mutant at 5 dpf. H shows an enlarged cross section; red arrowhead indicates a dynein arm. Scale bars: 1 μm in G; 100 nm in H. (I) Cilia motility in scohi459 mutant and wild-type siblings at 5 dpf. Cilia with a beating frequency of between 36 and 55 Hz are categorized as fast, whereas cilia with frequency of between 21 and 35, and between 5 and 20 are categorized as medium and slow, respectively. mph, morphant; mut, mutant; wt, wild type.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
arl13b Ab1-arl13b WT standard conditions 20-25 somites pronephric duct cilium IHC
Ab1-arl13b WT + MO1-arl13b standard conditions 20-25 somites pronephric duct cilium IHC
cdh17 Ab1-cdh17 WT standard conditions Prim-15 pronephric duct IHC
Ab1-cdh17 Day 5 pronephric duct IHC
Ab1-cdh17 arl13bhi459Tg/hi459Tg standard conditions Prim-15 pronephric duct IHC
Ab1-cdh17 Day 5 pronephric duct IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC WT standard conditions 20-25 somites pronephric duct cilium
IHC Prim-15 pronephric duct cilium
IHC Day 5 pronephric duct cilium
IHC WT + MO1-arl13b standard conditions 20-25 somites pronephric duct cilium
IHC arl13bhi459Tg/hi459Tg standard conditions Prim-15 pronephric duct cilium
IHC Day 5 pronephric duct cilium
Phenotype Details
Fish Conditions Stage Phenotype
arl13bhi459Tg/hi459Tg standard conditions Prim-15 cilium assembly process quality, abnormal
Prim-15 pronephric duct cilium decreased amount, abnormal
Prim-15 pronephric duct cilium decreased length, abnormal
Day 5 epithelial cilium movement involved in extracellular fluid movement decreased rate, abnormal
Day 5 pronephric duct axoneme morphology, normal
Day 5 pronephric duct cilium disoriented, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development