FIGURE

Fig. 5

ID

ZDB-FIG-091217-77

Publication

Duldulao et al., 2009 - Cilia localization is essential for in vivo functions of the Joubert syndrome protein Arl13b/Scorpion

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Fig. 5

arl13b is required for proper left-right asymmetry. (A) In situ hybridization for cardiac myosin light chain (cmlc2) showing the heart tubes of wild-type and morphant embryos at 26 hpf. (B) Tabulation of heart position from three independent experiments. (C) In situ hybridization for southpaw on wild-type and morphant embryos at the 18- to 20-somite stage, in dorsal view with head to the top. (D,E) KV in a wild-type embryo (D) and a morphant (E). Scale bars: 20 μm. Ab, absent; Bi, bilateral; L, left-sided; M, middle; mph, morphant; R, right-sided; wt, wild type.

Expression Data

Genes:	myl7 spaw
Fish:	WT WT + MO1-arl13b
Knockdown Reagent:	MO1-arl13b
Anatomical Terms:	heart tube lateral plate mesoderm
Stage Range:	14-19 somites to Prim-5

Expression Detail

Fish	Conditions	Stage	Anatomy	Assay
WT	standard conditions	Prim-5	heart tube	ISH
WT + MO1-arl13b	standard conditions	Prim-5	heart tube	ISH
WT	standard conditions	14-19 somites	lateral plate mesoderm	ISH
WT + MO1-arl13b	standard conditions	14-19 somites	lateral plate mesoderm	ISH

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Antibody Labeling

No data available

Phenotype Data

Fish:	WT + MO1-arl13b
Knockdown Reagent:	MO1-arl13b
Observed In:	determination of left/right asymmetry in lateral mesoderm determination of left/right symmetry heart lateral plate mesoderm
Stage Range:	14-19 somites to Prim-5

Phenotype Detail

Acknowledgments

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