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ZFIN ID: ZDB-FIG-091217-77
Duldulao et al., 2009 - Cilia localization is essential for in vivo functions of the Joubert syndrome protein Arl13b/Scorpion. Development (Cambridge, England)   136(23):4033-4042 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 14-19 somites to Prim-5

Fig. 5 arl13b is required for proper left-right asymmetry. (A) In situ hybridization for cardiac myosin light chain (cmlc2) showing the heart tubes of wild-type and morphant embryos at 26 hpf. (B) Tabulation of heart position from three independent experiments. (C) In situ hybridization for southpaw on wild-type and morphant embryos at the 18- to 20-somite stage, in dorsal view with head to the top. (D,E) KV in a wild-type embryo (D) and a morphant (E). Scale bars: 20 μm. Ab, absent; Bi, bilateral; L, left-sided; M, middle; mph, morphant; R, right-sided; wt, wild type.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myl7 WT standard conditions Prim-5 heart tube ISH
WT + MO1-arl13b standard conditions Prim-5 heart tube ISH
spaw WT standard conditions 14-19 somites lateral plate mesoderm ISH
WT + MO1-arl13b standard conditions 14-19 somites lateral plate mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-arl13b standard conditions 14-19 somites determination of left/right symmetry process quality, abnormal
14-19 somites heart bilateral symmetry, abnormal
Prim-5 determination of left/right asymmetry in lateral mesoderm process quality, abnormal
Prim-5 lateral plate mesoderm bilateral symmetry, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development