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ZFIN ID: ZDB-FIG-091217-74
Duldulao et al., 2009 - Cilia localization is essential for in vivo functions of the Joubert syndrome protein Arl13b/Scorpion. Development (Cambridge, England)   136(23):4033-4042 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Term:
Stage Range: 4-cell to Day 5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 5-9 somites

Fig. 2 hi459 is a zygotic null allele of arl13b (sco). (A) arl13b transcripts in embryos at 5 dpf shown by RT-PCR of cDNA from hi459 mutant embryos (lanes 2, 4, 6) and wild-type siblings (lanes 1, 3, 5). m, size marker; lanes 1-2, RT-PCR with a pair of primers spanning the proviral insertion site in arl13b; lanes 3-4, RT-PCR with a pair of primers for beta-actin as loading controls; lanes 5-6, RT-PCR with a pair of primers 3′ to the proviral insertion site in arl13b. (B) Absence of Arl13b in hi459 mutant embryos at 5 dpf. A band of the predicted size can be seen in samples immunoprecipitated with anti-Sco from lysate of wild-type siblings (w), but not in the sample from hi459 mutant embryos (m), nor in the lysate precipitated with protein A beads alone. (C) RT-PCR time-course for arl13b expression. 4-32, four- to 32-cell stage; 256-1k, 256- to 1000-cell stage; 30%, 30% epiboly. (D) Presence of Arl13b in embryos at the 64-cell stage. Lysates from embryos at the 64-cell stage were subjected to immunoprecipitation using anti-Sco (Sco) or pre-immune IgG (Pre) and precipitated proteins detected by western analysis using anti-Sco. (E-H) Wild type (wt) and arl13b morphant (mph) embryos in dorsal view (E,F) and side view (G,H) at around the 8-somite stage. Arrows in E and F point to somites. In G and H, body gap angle was labeled using ImageJ. (I) Gap angle in degrees in wild type (wt, n=13) embryos and arl13b morphants (mo, n=14; *P<0.001). (J) Gap angle in degrees in arl13b morphants co-injected with eGFP (eGFP, n=13) or sco mRNA (sco, n=15; *P<0.001).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
arl13b WT standard conditions 4-cell to Day 5 whole organism RTPCR
Ab1-arl13b 64-cell whole organism WB
Ab1-arl13b Day 5 whole organism WB
Ab1-arl13b arl13bhi459Tg/hi459Tg standard conditions Day 5 Not Detected whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-arl13b standard conditions 5-9 somites axis decreased length, abnormal
5-9 somites convergent extension involved in gastrulation process quality, abnormal
5-9 somites somite increased width, abnormal
5-9 somites trunk increased width, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development