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ZFIN ID: ZDB-FIG-091217-74
Duldulao et al., 2009 - Cilia localization is essential for in vivo functions of the Joubert syndrome protein Arl13b/Scorpion. Development (Cambridge, England)   136(23):4033-4042 Full text @ Development
Anatomical Term:
Stage Range: 4-cell to Day 5
Knockdown Reagent:
Observed In:
Stage: 5-9 somites

Fig. 2 hi459 is a zygotic null allele of arl13b (sco). (A) arl13b transcripts in embryos at 5 dpf shown by RT-PCR of cDNA from hi459 mutant embryos (lanes 2, 4, 6) and wild-type siblings (lanes 1, 3, 5). m, size marker; lanes 1-2, RT-PCR with a pair of primers spanning the proviral insertion site in arl13b; lanes 3-4, RT-PCR with a pair of primers for beta-actin as loading controls; lanes 5-6, RT-PCR with a pair of primers 3′ to the proviral insertion site in arl13b. (B) Absence of Arl13b in hi459 mutant embryos at 5 dpf. A band of the predicted size can be seen in samples immunoprecipitated with anti-Sco from lysate of wild-type siblings (w), but not in the sample from hi459 mutant embryos (m), nor in the lysate precipitated with protein A beads alone. (C) RT-PCR time-course for arl13b expression. 4-32, four- to 32-cell stage; 256-1k, 256- to 1000-cell stage; 30%, 30% epiboly. (D) Presence of Arl13b in embryos at the 64-cell stage. Lysates from embryos at the 64-cell stage were subjected to immunoprecipitation using anti-Sco (Sco) or pre-immune IgG (Pre) and precipitated proteins detected by western analysis using anti-Sco. (E-H) Wild type (wt) and arl13b morphant (mph) embryos in dorsal view (E,F) and side view (G,H) at around the 8-somite stage. Arrows in E and F point to somites. In G and H, body gap angle was labeled using ImageJ. (I) Gap angle in degrees in wild type (wt, n=13) embryos and arl13b morphants (mo, n=14; *P<0.001). (J) Gap angle in degrees in arl13b morphants co-injected with eGFP (eGFP, n=13) or sco mRNA (sco, n=15; *P<0.001).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
arl13b WT standard conditions 4-cell to Day 5 whole organism RTPCR
Ab1-arl13b 64-cell whole organism WB
Ab1-arl13b Day 5 whole organism WB
Ab1-arl13b arl13bhi459Tg/hi459Tg standard conditions Day 5 Not Detected whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-arl13b standard conditions 5-9 somites axis decreased length, abnormal
5-9 somites convergent extension involved in gastrulation process quality, abnormal
5-9 somites somite increased width, abnormal
5-9 somites trunk increased width, abnormal
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development