ZFIN ID: ZDB-FIG-091214-11
Bonkowsky et al., 2008 - Domain-specific regulation of foxP2 CNS expression by lef1. BMC Developmental Biology   8:103 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-25

Fig. 3 Loss of lef1 leads to absent foxP2 expression in the tectum, mid-hindbrain boundary, and hindbrain. Whole-mount in situs at 36 hpf; anterior to left, dorsal up, eyes removed. Scale bar = 50 μm. (Conditions: hs:Δtcf, Tg(hsp70l:Δtcf-GFP)w26; lef1 mo, lef1 morphants; wt, wild type; x8, homozygous Df(LG01)x8. Abbreviations: hb, hindbrain; mhb, mid-hindbrain boundary; tec, tectum; tel, telencephalon.) (A, B) lef1 morphant (B) lacks expression in tectum, MHB, and hindbrain (arrows), compared to wild-type (A). (C) Df(LG01)x8 homozygote lacks expression in tectum, MHB, and hindbrain (arrows). (D) Tg(hsp70l:Δtcf:GFP)w26 embryo, three hours post-heat shock, lacks expression in tectum, MHB, and hindbrain (arrows). (E, F) mbx in situs; staining in tectum and hindbrain is indistinguishable between wild type and lef1 morphants (F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dmbx1a WT control Prim-25 hindbrain ISH
Prim-25 optic tectum ISH
WT + MO2-lef1 standard conditions Prim-25 hindbrain ISH
Prim-25 optic tectum ISH
foxp2 Df(Chr01:lef1,msxb)x8/x8 control Prim-25 Not Detected hindbrain ISH
Prim-25 Not Detected midbrain hindbrain boundary ISH
Prim-25 Not Detected optic tectum ISH
WT control Prim-25 hindbrain ISH
Prim-25 midbrain hindbrain boundary ISH
Prim-25 optic tectum ISH
Prim-25 telencephalon ISH
WT + MO2-lef1 standard conditions Prim-25 Not Detected hindbrain ISH
Prim-25 Not Detected midbrain hindbrain boundary ISH
Prim-25 Not Detected optic tectum ISH
Prim-25 telencephalon ISH
w26Tg heat shock Prim-25 Not Detected hindbrain ISH
Prim-25 Not Detected midbrain hindbrain boundary ISH
Prim-25 Not Detected optic tectum ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.