FIGURE

Fig. 6

ID

ZDB-FIG-091214-13

Publication

Bonkowsky et al., 2008 - Domain-specific regulation of foxP2 CNS expression by lef1

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Fig. 6

lef1 is necessary for expression from foxP2-enhancerA.1, foxP2-enhancerB, and foxP2-enhancerD. Whole-mount gfp in situs at 36 hpf; anterior to left, dorsal up, eyes removed. Scale bar = 50 μm. Conditions (wild type, wt; or lef1 morphant, lef1 MO) are shown above the panels, enhancer names to left. (Abbreviations: dd, dorsal diencephalon; hb, hindbrain; tec, tectum; tel, telencephalon.) (A) Tg(foxP2-enhancerA.1:EGFP)^zc44expresses in telencephalon, tectum, and hindbrain. (B) Tg(foxP2-enhancerA.1:EGFP)^zc44embryo injected with lef1 morpholino lacks GFP expression in tectum and hindbrain (arrows), although telencephalic expression persists. (C) Tg(foxP2-enhancerB:EGFP)^zc41expresses in telencephalon and hindbrain. (D) Tg(foxP2-enhancerB:EGFP)^zc41embryo injected with lef1 morpholino lacks GFP expression in the hindbrain (arrow), but telencephalic expression is present. (E) Tg(foxP2-enhancerD:EGFP)^zc47expresses in dorsal diencephalon and tectum. (F) Tg(foxP2-enhancerD:EGFP)^zc47embryo injected with lef1 morpholino lacks GFP expression in the tectum (arrow), but dorsal diencephalic expression persists. (G) Tg(elavl3:EGFP)^zf8embryo shows GFP expression in all post-mitotic neurons. (H) Tg(elavl3:EGFP)^zf8embryo injected with lef1 morpholino still has GFP expression in the tectum and hindbrain.

Expression Data

Gene:	EGFP
Fish:	knu3Tg zc41Tg zc44Tg zc47Tg knu3Tg + MO2-lef1 zc41Tg + MO2-lef1 zc44Tg + MO2-lef1 zc47Tg + MO2-lef1
Knockdown Reagent:	MO2-lef1
Anatomical Terms:	diencephalon hindbrain midbrain hindbrain boundary neuron optic tectum telencephalon
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.