FIGURE

Fig. S2

ID

ZDB-FIG-091214-16

Publication

Bonkowsky et al., 2008 - Domain-specific regulation of foxP2 CNS expression by lef1

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Fig. S2

lef1 is necessary for expression from foxP2-enhancerA.1, foxP2-enhancerB, and foxP2-enhancerD. Whole-mount confocal images at 52 hpf (except foxP2-enhancerD), lateral view, anterior left, dorsal up. Conditions (wild type- wt, or lef1 morphant- lef1 MO) are shown above the panels, while the enhancer name is to the left of the panels. (Abbreviations: hb: hindbrain; tec: tectum; tel: telencephalon.) (A) Tg(foxP2-enhancerA.1:EGFP)^zc44expresses in telencephalon, tectum, and hindbrain. (B) Tg(foxP2-enhancerA.1:EGFP)^zc44embryo injected with lef1 morpholino: GFP expression is absent in the tectum and hindbrain (arrows), although telencephalic expression persists. (C) Tg(foxP2-enhancerB:EGFP)^zc41expresses in telencephalon, tectum, and hindbrain. (D) Tg(foxP2-enhancerB:EGFP)^zc41embryo injected with lef1 morpholino: GFP expression is absent in the hindbrain (arrow), but still present in the telencephalon. (E) Tg(foxP2-enhancerD:EGFP)^zc47expresses in tectum. (F) Tg(foxP2-enhancerD:EGFP)^zc47embryo injected with lef1 morpholino: GFP expression is absent in the tectum (arrow). (G) Tg(elavl3:EGFP)^zf8embryo shows GFP expression in all post-mitotic neurons. (H) Tg(elavl3:EGFP)^zf8embryo injected with lef1 morpholino: GFP expression is still present in the tectum and hindbrain. (I) Tg(pax2a:GFP)^e1embryo shows expression in the MHB and hindbrain. (J) Tg(pax2a:GFP)^e1embryo injected with lef1 morpholino has persistent expression in the MHB and hindbrain.

Expression Data

Genes:	EGFP GFP
Fish:	e1Tg knu3Tg zc41Tg zc44Tg zc47Tg e1Tg + MO2-lef1 knu3Tg + MO2-lef1 zc41Tg + MO2-lef1 zc44Tg + MO2-lef1 zc47Tg + MO2-lef1
Knockdown Reagent:	MO2-lef1
Anatomical Terms:	hindbrain midbrain hindbrain boundary neuron optic tectum telencephalon
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.