ZFIN ID: ZDB-FIG-091214-16
Bonkowsky et al., 2008 - Domain-specific regulation of foxP2 CNS expression by lef1. BMC Developmental Biology   8:103 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec

Fig. S2 lef1 is necessary for expression from foxP2-enhancerA.1, foxP2-enhancerB, and foxP2-enhancerD. Whole-mount confocal images at 52 hpf (except foxP2-enhancerD), lateral view, anterior left, dorsal up. Conditions (wild type- wt, or lef1 morphant- lef1 MO) are shown above the panels, while the enhancer name is to the left of the panels. (Abbreviations: hb: hindbrain; tec: tectum; tel: telencephalon.) (A) Tg(foxP2-enhancerA.1:EGFP)zc44 expresses in telencephalon, tectum, and hindbrain. (B) Tg(foxP2-enhancerA.1:EGFP)zc44 embryo injected with lef1 morpholino: GFP expression is absent in the tectum and hindbrain (arrows), although telencephalic expression persists. (C) Tg(foxP2-enhancerB:EGFP)zc41 expresses in telencephalon, tectum, and hindbrain. (D) Tg(foxP2-enhancerB:EGFP)zc41 embryo injected with lef1 morpholino: GFP expression is absent in the hindbrain (arrow), but still present in the telencephalon. (E) Tg(foxP2-enhancerD:EGFP)zc47 expresses in tectum. (F) Tg(foxP2-enhancerD:EGFP)zc47 embryo injected with lef1 morpholino: GFP expression is absent in the tectum (arrow). (G) Tg(elavl3:EGFP)zf8 embryo shows GFP expression in all post-mitotic neurons. (H) Tg(elavl3:EGFP)zf8 embryo injected with lef1 morpholino: GFP expression is still present in the tectum and hindbrain. (I) Tg(pax2a:GFP)e1 embryo shows expression in the MHB and hindbrain. (J) Tg(pax2a:GFP)e1 embryo injected with lef1 morpholino has persistent expression in the MHB and hindbrain.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP knu3Tg control Long-pec hindbrain IFL
Long-pec neuron IFL
Long-pec optic tectum IFL
knu3Tg + MO2-lef1 standard conditions Long-pec hindbrain IFL
Long-pec neuron IFL
Long-pec optic tectum IFL
zc41Tg control Long-pec hindbrain IFL
Long-pec telencephalon IFL
zc41Tg + MO2-lef1 standard conditions Long-pec Not Detected hindbrain IFL
Long-pec telencephalon IFL
zc44Tg control Long-pec hindbrain IFL
Long-pec optic tectum IFL
Long-pec telencephalon IFL
zc44Tg + MO2-lef1 standard conditions Long-pec Not Detected hindbrain IFL
Long-pec Not Detected optic tectum IFL
Long-pec telencephalon IFL
zc47Tg control Long-pec optic tectum IFL
zc47Tg + MO2-lef1 standard conditions Long-pec Not Detected optic tectum IFL
GFP e1Tg control Long-pec hindbrain IFL
Long-pec midbrain hindbrain boundary IFL
e1Tg + MO2-lef1 standard conditions Long-pec hindbrain IFL
Long-pec midbrain hindbrain boundary IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.