ZFIN ID: ZDB-FIG-091214-15
Bonkowsky et al., 2008 - Domain-specific regulation of foxP2 CNS expression by lef1. BMC Developmental Biology   8:103 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec

Fig. S1 Loss of lef1 leads to absent foxP2 expression in the tectum, mid-hindbrain boundary, and hindbrain. Whole-mount in situs at 48 hpf; anterior to left, dorsal up, eyes removed. Scale bar = 50 μm. (Conditions: hs:Δtcf- Tg(hsp70l:Δtcf:GFP)w26, lef1 mo- lef1 morphants, wt- wild type, x8- homozygous Df(LG01)x8. Abbreviations: hb: hindbrain; mhb: mid-hindbrain boundary; tec: tectum; tel: telencephalon.) (A, B) lef1 morphant (B) lacks expression in tectum and has diminished expression in the hindbrain (arrows), compared to wild-type (A). (C) In Df(LG01)x8 homozygote, expression is absent in the tectum and hindbrain (arrows). (D) In Tg(hsp70l:Δtcf:GFP)w26 embryo, collected four hours after heat shocking, expression is absent from the tectum and hindbrain (arrows). MHB expression is reduced but not absent, perhaps because lef1 induction of foxP2 expression is already sufficiently established by the time of heat-shock.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
foxp2 Df(Chr01:lef1,msxb)x8/x8 control Long-pec Not Detected hindbrain ISH
Long-pec Not Detected optic tectum ISH
WT control Long-pec hindbrain ISH
Long-pec optic tectum ISH
Long-pec telencephalon ISH
WT + MO2-lef1 standard conditions Long-pec hindbrain ISH
Long-pec Not Detected optic tectum ISH
Long-pec telencephalon ISH
w26Tg heat shock Long-pec Not Detected hindbrain ISH
Long-pec Not Detected optic tectum ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.