ZFIN ID: ZDB-FIG-091208-3
Gosse et al., 2009 - An essential role for Radar (Gdf6a) in inducing dorsal fate in the zebrafish retina. Proceedings of the National Academy of Sciences of the United States of America   106(7):2236-2241 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Term:
Stage: Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage: Days 7-13

Fig. 1

Morphological and retinotectal phenotypes of radars327. (A) Zebrafish radars327 mutants have small eyes and appear dark, because of a VBA defect (see text). Dorsal brightfield views of 7 dpf WT sibling and homozygous radars327 mutant larvae. (B) radars327 mutants lack ventral innervation of the optic tectum. Dorsal confocal projections of 7 dpf larvae show that innervating RGC axons (expressing Brn3c:mGFP) are confined to the dorsal tectum in the mutant. Costaining with a neuropil marker (zrf-3 antibody) reveals that the size of the tectum is similar in WT and mutant. (C) radars327 mutants have a compressed dorsal–ventral retinotectal map. Fixed WT and radars327 eyes (7 dpf) were injected with DiO (ventrally) and DiI (dorsally). Lateral confocal projections are shown. Arrow highlights ventral tectal region not innervated by RGCs in radars327; asterisks show positions of skin melanophores. (Scale bars: 300 μm in A, 100 μm in B and C.)

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zrf-3 IHC gdf6as327/s327; s356tTg standard conditions Days 7-13 optic tectum
IHC s356tTg standard conditions Days 7-13 optic tectum
Phenotype Details
Fish Conditions Stage Phenotype
gdf6as327/s327 standard conditions Days 7-13 eye decreased size, abnormal
Days 7-13 optic tectum has fewer parts of type retinal ganglion cell neuron projection, abnormal
Days 7-13 retinal ganglion cell axon guidance process quality, abnormal
gdf6as327/s327; s356tTg standard conditions Days 7-13 optic tectum has fewer parts of type retinal ganglion cell neuron projection, abnormal
Days 7-13 optic tectum size, normal
Days 7-13 retinal ganglion cell axon guidance disrupted, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA