ZFIN Image: Gosse et al., 2009, Fig. 1

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Figure Caption

Fig. 1

Morphological and retinotectal phenotypes of radar^s327. (A) Zebrafish radar^s327 mutants have small eyes and appear dark, because of a VBA defect (see text). Dorsal brightfield views of 7 dpf WT sibling and homozygous radar^s327 mutant larvae. (B) radar^s327 mutants lack ventral innervation of the optic tectum. Dorsal confocal projections of 7 dpf larvae show that innervating RGC axons (expressing Brn3c:mGFP) are confined to the dorsal tectum in the mutant. Costaining with a neuropil marker (zrf-3 antibody) reveals that the size of the tectum is similar in WT and mutant. (C) radar^s327 mutants have a compressed dorsal–ventral retinotectal map. Fixed WT and radar^s327 eyes (7 dpf) were injected with DiO (ventrally) and DiI (dorsally). Lateral confocal projections are shown. Arrow highlights ventral tectal region not innervated by RGCs in radar^s327; asterisks show positions of skin melanophores. (Scale bars: 300 μm in A, 100 μm in B and C.)

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA