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ZFIN ID: ZDB-FIG-090831-3
Insinna et al., 2009 - Different roles for KIF17 and kinesin II in photoreceptor development and maintenance. Developmental dynamics : an official publication of the American Association of Anatomists   238(9):2211-2222 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 2 Disruption of kif3B expression by an antisense morpholino oligonucleotide. A: (Upper panel) Images of 3-day-old larvae that survived an injection of 500 μM (AKIF3B 500 μM) or 250 μM (AKIF3B 250 μM) of the translation-blocking morpholino. (Middle panel) A control injected embryo. (Lower panel) Three-day-old larva after injection with 250 μM of a splice-blocking morpholino (SpKIF3B). B: Western blots of whole embryo extracts showing the reduction of KIF3B and KIF3A in AKIF3B morphants at 3 days postfertilization (dpf); anti-KIF17 and anti-γ-tubulin were used as controls. C: Western blot of three separate whole SpKif3B (250 μM) morphant and control embryo extracts showing similar depletion of KIF3B and KIF3A as observed in translation-blocking morphants (B above); anti-γ-tubulin was used as a loading control. D: Semi-thin section of the eye of an AKIF3B (250 μM) morphant at 3 dpf. Insets: Electron microscopy (EM) views of AKIF3B (250 μM) photoreceptors. E: Semi-thin section of the eye of an AKIF3B (500 μM) morphant at 3 dpf. Note the absence of retinal lamination and photoreceptor differentiation. Scale bar = 10 μm in D,E, 2.5 μm in insets.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
kif3a Ab1-kif2 WT control Protruding-mouth whole organism WB
Ab1-kif2 WT standard conditions Protruding-mouth whole organism WB
Ab1-kif2 WT + MO1-kif3b standard conditions Protruding-mouth whole organism WB
Ab1-kif2 WT + MO2-kif3b standard conditions Protruding-mouth whole organism WB
kif3b Ab1-kif2 WT control Protruding-mouth whole organism WB
Ab1-kif2 WT standard conditions Protruding-mouth whole organism WB
Ab1-kif2 WT + MO1-kif3b standard conditions Protruding-mouth whole organism WB
Ab1-kif2 WT + MO2-kif3b standard conditions Protruding-mouth whole organism WB
kif17 Ab1-kif17 WT + MO1-kif3b standard conditions Protruding-mouth whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB/TU + MO1-kif3b standard conditions Protruding-mouth brain hydrocephalic, abnormal
Protruding-mouth eye decreased size, abnormal
Protruding-mouth optic stalk morphology, abnormal
Protruding-mouth pericardium edematous, abnormal
Protruding-mouth photoreceptor cell differentiation disrupted, abnormal
Protruding-mouth photoreceptor outer segment layer aplastic, abnormal
Protruding-mouth retina development in camera-type eye delayed, abnormal
Protruding-mouth retina layer formation disrupted, abnormal
Protruding-mouth retinal neural layer morphology, abnormal
Protruding-mouth whole organism curved, abnormal
Protruding-mouth whole organism lethal (sensu genetics), abnormal
AB/TU + MO2-kif3b standard conditions Protruding-mouth brain hydrocephalic, abnormal
Protruding-mouth eye decreased size, abnormal
Protruding-mouth pericardium edematous, abnormal
Protruding-mouth whole organism curved, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.