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ZFIN ID: ZDB-FIG-090501-2
Row et al., 2009 - Bmp inhibition is necessary for post-gastrulation patterning and morphogenesis of the zebrafish tailbud. Developmental Biology   329(1):55-63 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 1-4 somites to 10-13 somites

Fig. 1 Bmp signaling is tightly regulated in the tailbud during early somitogenesis. (A, B) Bmp signaling is active in the mesodermal progenitor zone. Expression of no tail is shown by FISH in green in 3-somite stage embryos marking both the mesodermal progenitor zone and the notochord (n). Bmp signaling is indicated by antibody staining for phosphorylated Smad proteins (red in B–D). Bmp inhibitors are expressed just anterior to the activated Smad zone. The Bmp inhibitors chordin, noggin1 and noggin2 are shown by FISH in green and the Smad staining is shown in red in 3-somite (C) and 12-somite (D) embryos. All embryos are shown from a dorsal view with anterior to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
chrd WT standard conditions 1-4 somites notochord ISH
1-4 somites segmental plate ISH
10-13 somites notochord ISH
10-13 somites segmental plate ISH
nog1 WT standard conditions 1-4 somites notochord ISH
1-4 somites segmental plate ISH
10-13 somites notochord ISH
10-13 somites segmental plate ISH
nog2 WT standard conditions 1-4 somites notochord ISH
1-4 somites segmental plate ISH
10-13 somites notochord ISH
10-13 somites segmental plate ISH
tbxta WT standard conditions 1-4 somites notochord ISH
1-4 somites tail bud presumptive mesoderm ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-smad IHC WT standard conditions 1-4 somites tail bud
IHC 10-13 somites tail bud
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 329(1), Row, R., and Kimelman, D., Bmp inhibition is necessary for post-gastrulation patterning and morphogenesis of the zebrafish tailbud, 55-63, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.