PUBLICATION
Bmp inhibition is necessary for post-gastrulation patterning and morphogenesis of the zebrafish tailbud
- Authors
- Row, R., and Kimelman, D.
- ID
- ZDB-PUB-090302-17
- Date
- 2009
- Source
- Developmental Biology 329(1): 55-63 (Journal)
- Registered Authors
- Kimelman, David, Row, Richard H.
- Keywords
- Bmp, Wnt, Mesoderm progenitors, Somitogenesis
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Body Patterning/genetics
- Body Patterning/physiology*
- Bone Morphogenetic Proteins/antagonists & inhibitors*
- Bone Morphogenetic Proteins/genetics
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Gastrulation*
- Gene Expression Regulation, Developmental
- Immunohistochemistry
- In Situ Hybridization
- Mesoderm/physiology
- Models, Genetic
- Morphogenesis/genetics
- Morphogenesis/physiology*
- Signal Transduction/physiology
- Tail/embryology
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 19236859 Full text @ Dev. Biol.
Citation
Row, R., and Kimelman, D. (2009) Bmp inhibition is necessary for post-gastrulation patterning and morphogenesis of the zebrafish tailbud. Developmental Biology. 329(1):55-63.
Abstract
Intricate interactions between the Wnt and Bmp signaling pathways pattern the gastrulating vertebrate embryo using a network of secreted protein ligands and inhibitors. While many of these proteins are expressed post-gastrula, their later roles have typically remained unclear, obscured by the effects of early perturbation. We find that Bmp signaling continues during somitogenesis in zebrafish embryos, with high activity in a small region of the mesodermal progenitor zone at the posterior end of the embryo. To test the hypothesis that Bmp inhibitors expressed just anterior to the tailbud are important to restrain Bmp signaling we produced a new zebrafish transgenic line, allowing temporal cell-autonomous activation of Bmp signaling and thereby bypassing the effects of the Bmp inhibitors. Ectopic activation of Bmp signaling during somitogenesis results in severe defects in the tailbud, including altered morphogenesis and gene expression. We show that these defects are due to non-autonomous effects on the tailbud, and present evidence that the tailbud defects are caused by alterations in Wnt signaling. We present a model in which the posteriorly expressed Bmp inhibitors function during somitogenesis to constrain Bmp signaling in the tailbud in order to allow normal expression of Wnt inhibitors in the presomitic mesoderm, which in turn constrain the levels of canonical and non-canonical Wnt signaling in the tailbud.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping