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ZFIN ID: ZDB-FIG-090127-10
Noël et al., 2008 - Organ-specific requirements for Hdac1 in liver and pancreas formation. Developmental Biology   322(2):237-250 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 3 Hdac1 is required for timely liver specification and differentiation. (A–J) In situ hybridisation analyses of hhex, prt/wnt2bb and cp expression in sibling and hdac1 mutant embryos; dorsal views, anterior to the top. (A, B) Wild type embryos express hhex in hepatoblasts at 24 hpf (bracket, A) and 48 hpf (arrowhead, B). Additionally, hhex is expressed in pancreatic tissue (asterisk). (C) hdac1 mutants lack hepatic hhex, though retain pancreatic expression at 24 hpf. At 48 hpf hhex expression is present in the liver of hdac1 mutants, although in a reduced domain (arrowhead, D). (E, F) Wild type embryos express prt/wnt2bb in the LPM at 26 hpf (bracket, E), however hdac1 mutants show reduced prt/wnt2bb expression (bracket, F). (G–J) cp is expressed in differentiating hepatocytes at 48 hpf and 4 dpf in wild type embryos (arrowheads, G, H). hdac1 mutants lack cp expression at 48 hpf (I); a subset of embryos express cp at 4 dpf (arrowhead, J). (K–N) Ventral projection of confocal stacks. Analysis of Tg(gutGFP)s854 embryos (K, M; GFP not shown) and Tg(lfabp:dsRed;elastaseA:eGFP) embryos (L, N) stained for 2F11 (blue) and Prox1 (red) reveals that in wild type embryos 2F11 expression is heightened in the hepatopancreatic ducts at 48 hpf (arrowhead, K) and 4 dpf (arrowhead L). hdac1 mutant embryos express 2F11 throughout the organ-forming region at 48 hpf (M), however expression is heightened in the duct at 4 dpf (N). In addition, wild type embryos express dsRed in the liver and GFP in the pancreas at 3 and 4 dpf (L) while only a subset of hdac1 mutants express dsRed and GFP at 4 dpf (N).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cp hdac1s436/s436 standard conditions Long-pec Not Detected hepatocyte ISH
Day 4 hepatocyte ISH
hdac1s436 standard conditions Long-pec hepatocyte ISH
Day 4 hepatocyte ISH
DsRed gz15Tg standard conditions Day 4 liver IFL
hdac1s436/s436; gz15Tg standard conditions Day 4 liver IFL
EGFP gz15Tg standard conditions Day 4 pancreas IFL
hdac1s436/s436; gz15Tg standard conditions Day 4 pancreas IFL
hhex hdac1s436/s436 standard conditions Prim-5 Not Detected liver primordium ISH
Prim-5 pancreatic bud ISH
Long-pec liver ISH
Long-pec pancreas ISH
hdac1s436 standard conditions Prim-5 liver primordium ISH
Prim-5 pancreatic bud ISH
Long-pec liver ISH
Long-pec pancreas ISH
prox1a Ab3-prox1 WT standard conditions Long-pec liver IHC
Ab3-prox1 hdac1s436/s436 standard conditions Long-pec liver IHC
wnt2bb hdac1s436/s436 standard conditions Prim-5 lateral plate mesoderm ISH
hdac1s436 standard conditions Prim-5 lateral plate mesoderm ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-2F11 IHC WT standard conditions Long-pec pancreatic duct
IHC gz15Tg standard conditions Day 4 pancreatic duct
IHC hdac1s436/s436 standard conditions Long-pec pancreatic duct
IHC hdac1s436/s436; gz15Tg standard conditions Day 4 pancreatic duct
Phenotype Details
Fish Conditions Stage Phenotype
hdac1s436/s436; gz4Tg standard conditions Day 4 liver morphology, abnormal
Day 4 pancreas morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 322(2), Noël, E.S., Casal-Sueiro, A., Busch-Nentwich, E., Verkade, H., Dong, P.D., Stemple, D.L., and Ober, E.A., Organ-specific requirements for Hdac1 in liver and pancreas formation, 237-250, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.