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ZFIN ID: ZDB-FIG-090127-12
Noël et al., 2008 - Organ-specific requirements for Hdac1 in liver and pancreas formation. Developmental Biology   322(2):237-250 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Fig. 5 Histone deacetylase function is required during late somitogenesis for liver specification and differentiation. TSA was applied at various time points, and digestive tract morphogenesis, liver specification and differentiation were examined by foxA1, hhex, prt/wnt2bb, and cp expression, respectively. 600 nM TSA produced the closest phenocopy of the hdac1s436 phenotype when assessed by foxA1 expression (compare panel B and C). This concentration was used in subsequent experiments. Horizontal bars show an expansion of the digestive tract in both TSA treated and hdac1 mutants when compared to wild type embryos (compare panels B, C with A). (D–I) Hepatoblast specification in TSA treated embryos was assessed by hhex expression. Application of TSA at 14 hpf resulted in an absence of hepatic hhex expression at both 24 and 48 hpf (E, H). However, treatment at 18 hpf resulted in wild type expression of hepatic hhex at 24 hpf (bracket, F), although strongly reduced at 48 hpf (arrowhead, I). (J–L) prt/wnt2bb expression was assessed in TSA treated embryos. TSA treatment at 16 hpf resulted in an absence of prt/wnt2bb expression in the LPM abutting the organ-forming endoderm (K), however application of TSA at 18 hpf resulted in wild type-like expression of prt/wnt2bb (bracket, L). (M–O) Hepatocyte differentiation in TSA treated embryos was assessed by cp expression. Embryos treated with TSA at 14 hpf showed no cp expression at 48 hpf (N), however embryos treated at 18 hpf express cp, but in a reduced domain (arrow, O). DMSO was added as a control in all cases (A, D, G, J, M), asterisk indicates position of the pancreas. Anterior to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cp WT control Long-pec hepatocyte ISH
WT chemical treatment: trichostatin A Long-pec hepatocyte ISH
WT chemical treatment: trichostatin A Long-pec Not Detected hepatocyte ISH
foxa1 WT control Prim-25 digestive system ISH
Prim-25 endoderm ISH
Prim-25 liver ISH
WT chemical treatment: trichostatin A Prim-25 digestive system ISH
Prim-25 endoderm ISH
Prim-25 liver ISH
hdac1s436/s436 standard conditions Prim-25 digestive system ISH
Prim-25 endoderm ISH
Prim-25 liver ISH
hhex WT control Prim-25 hepatocyte ISH
Long-pec hepatocyte ISH
WT chemical treatment: trichostatin A Prim-25 hepatocyte ISH
Long-pec hepatocyte ISH
WT chemical treatment: trichostatin A Prim-25 Not Detected hepatocyte ISH
Long-pec Not Detected hepatocyte ISH
wnt2bb WT control Prim-5 lateral plate mesoderm ISH
WT chemical treatment: trichostatin A Prim-5 Not Detected lateral plate mesoderm ISH
WT chemical treatment: trichostatin A Prim-5 lateral plate mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 322(2), Noël, E.S., Casal-Sueiro, A., Busch-Nentwich, E., Verkade, H., Dong, P.D., Stemple, D.L., and Ober, E.A., Organ-specific requirements for Hdac1 in liver and pancreas formation, 237-250, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.