FIGURE

Fig. 4

ID

ZDB-FIG-090109-15

Publication

Linville et al., 2009 - Combinatorial roles for zebrafish retinoic acid receptors in the hindbrain, limbs and pharyngeal arches

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Fig. 4

Depletion of rarga alone disrupts posterior pharyngeal arches. Lateral views, anterior to the left. (A, D) Controls. (B, E) rarga morphants (C, F) rarga/rargb double morphants. (A–C) Whole mount in situ hybridization to detect dlx2 mRNA reveals reductions in migrating neural crest cells in arches 3–5 (arrows) in both single (B; 28/30) and double (C; 35/35) morphants. (D–F) Whole mount, immunostaining with the zn-5 antibody reveals reductions in the pharyngeal pouch endoderm (particularly pouch #5, arrowheads) in these arches, but no defects in zn5+ cells in the hindbrain or cranial ganglia in single (E) and double (F) morphants. Abbreviations: e, eye; m, mandibular; h, hyoid; ot, otic vesicle.

Expression Data

Genes:	alcama dlx2a
Antibody:	zn-5
Fish:	WT WT + MO1-rarga WT + MO1-rarga + MO1-rargb + MO1-tp53
Knockdown Reagents:	MO1-rarga MO1-rargb MO1-tp53
Anatomical Terms:	mandibular arch skeleton pharyngeal arch 2 pharyngeal arch 3 pharyngeal arch 4 pharyngeal arch 5 pharyngeal pouch 1 endoderm pharyngeal pouch 2 endoderm pharyngeal pouch 3 endoderm pharyngeal pouch 4 endoderm pharyngeal pouch 5 endoderm
Stage Range:	Prim-15 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-rarga WT + MO1-rarga + MO1-rargb + MO1-tp53
Knockdown Reagents:	MO1-rarga MO1-rargb MO1-tp53
Observed In:	pharyngeal arch 5
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 325(1), Linville, A., Radtke, K., Waxman, J.S., Yelon, D., and Schilling, T.F., Combinatorial roles for zebrafish retinoic acid receptors in the hindbrain, limbs and pharyngeal arches, 60-70, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.