ZFIN ID: ZDB-FIG-090109-18
Linville et al., 2009 - Combinatorial roles for zebrafish retinoic acid receptors in the hindbrain, limbs and pharyngeal arches. Developmental Biology   325(1):60-70 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 75%-epiboly to 14-19 somites

Fig. 7 rars are differentially regulated by RA. (A–I) Whole mount in situ hybridization for three of the four rars during gastrulation (8.5 hpf). Dorsal views, anterior to the top, of wild-types (A, D, G), AGN-treated (B, E, H), and RA-treated (C, F, I) embryos. Arrowheads indicate approximate borders of receptor expression along the anterior–posterior axis. (A–C) Only raraa expression is reduced by AGN treatment (B) and induced by RA (C). (D–F) AGN has no effect on rarab expression (E) though expression is upregulated anteriorly by RA (F). (G–I) AGN treatments also have little effect on rarga expression (H), but surprisingly RA treatments downregulate expression (I). (J–O) Expression of rars in the hindbrain at 18 hpf. Dorsal views, anterior to the left, of flat-mounted hindbrains showing rar expression (blue) and krox20 in r3 and r5 (red). Similar to the gastrula studies, only raraa expression is reduced in aldh1a2 mutants (K) while other receptors appear unaffected (M, O). Abbreviations: cm, cranial mesoderm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
raraa WT standard conditions 75%-epiboly ectoderm ISH
14-19 somites hindbrain ISH
14-19 somites neural tube ISH
WT chemical treatment: all-trans-retinoic acid 75%-epiboly ectoderm ISH
aldh1a2i26/i26 standard conditions 14-19 somites hindbrain ISH
14-19 somites neural tube ISH
rarab WT standard conditions 75%-epiboly ectoderm ISH
14-19 somites hindbrain ISH
14-19 somites neural tube ISH
14-19 somites pharyngeal arch ISH
WT chemical treatment: all-trans-retinoic acid 75%-epiboly ectoderm ISH
aldh1a2i26/i26 standard conditions 14-19 somites hindbrain ISH
14-19 somites neural tube ISH
14-19 somites pharyngeal arch ISH
rarga WT standard conditions 75%-epiboly epiblast ISH
75%-epiboly hypoblast ISH
75%-epiboly margin ISH
14-19 somites cranium mesoderm ISH
14-19 somites hindbrain ISH
WT chemical treatment: all-trans-retinoic acid 75%-epiboly epiblast ISH
75%-epiboly hypoblast ISH
75%-epiboly margin ISH
aldh1a2i26/i26 standard conditions 14-19 somites cranium mesoderm ISH
14-19 somites hindbrain ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 325(1), Linville, A., Radtke, K., Waxman, J.S., Yelon, D., and Schilling, T.F., Combinatorial roles for zebrafish retinoic acid receptors in the hindbrain, limbs and pharyngeal arches, 60-70, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.