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ZFIN ID: ZDB-FIG-090109-16
Linville et al., 2009 - Combinatorial roles for zebrafish retinoic acid receptors in the hindbrain, limbs and pharyngeal arches. Developmental Biology   325(1):60-70 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Day 4

Fig. 5 Depletion of rarab alone disrupts pectoral fin budding. (A–C) Alcian blue stained cartilage in larvae (4 dpf). Ventral views, anterior to the left. (A) In controls the pectoral fins protrude laterally just posterior to the skull. (B, C) Lack of fins and fin cartilages in both aldh1a2 mutants (B, arrow; N = 12) and rarab morphants (C, arrow; N = 24). (D–F) tbx5 expression at 32 hpf, detected by whole mount in situ hybridization. Dorsal views, anterior to the left. (D) In controls, tbx5 labels the bilateral pair of early pectoral fin buds, which are absent in aldh1a2 mutants (E, arrow) and rarab morphants (F, arrow; N = 7). (G, H) Live 96 hpf larvae, dorsal views, anterior to the left. rarab morphants occasionally form a small pectoral fin on one side (H, arrowhead). (K) Transverse sections at forelimb levels at 24 hpf reveal rarab expression in the neural tube and lateral plate mesoderm, but absent from the adjacent somite or intermediate mesoderm. Abbreviations: fb, fin bud; IM, intermediate mesoderm; LPM, lateral plate mesoderm; pf, pectoral fin; nc, notochord; nt, neural tube.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
rarab WT standard conditions Prim-5 Not Detected intermediate mesoderm ISH
Prim-5 lateral plate mesoderm ISH
Prim-5 neural tube ISH
Prim-5 Not Detected somite ISH
tbx5a WT control Prim-15 pectoral fin bud ISH
WT + MO1-rarab standard conditions Prim-15 Not Detected pectoral fin bud ISH
aldh1a2i26/i26 standard conditions Prim-15 Not Detected pectoral fin bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-rarab standard conditions Prim-15 pectoral fin bud aplastic, abnormal
Day 4 pectoral fin aplastic, abnormal
aldh1a2i26/i26 standard conditions Prim-15 pectoral fin bud aplastic, abnormal
Day 4 pectoral fin aplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 325(1), Linville, A., Radtke, K., Waxman, J.S., Yelon, D., and Schilling, T.F., Combinatorial roles for zebrafish retinoic acid receptors in the hindbrain, limbs and pharyngeal arches, 60-70, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.