ZFIN ID: ZDB-FIG-081007-18
Huang et al., 2008 - Mypt1-mediated spatial positioning of Bmp2-producing cells is essential for liver organogenesis. Development (Cambridge, England)   135(19):3209-3218 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage Range: 1-cell to Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. S5 Bmp signaling is essential for liver development in zebrafish. (A) Immunoblot analyses of dominant-negative BmpR1a-GFP expression using an anti-GFP antibody after heat-shock treatment. Total protein lysates were prepared from heat-shocked embryos at different time intervals as indicated. (B-D) WISH using the lfabp (B), prox1 (C) or ifabp (D) probes and F59 antibody (C) on heat-shocked embryos. Embryos in B and D were heat shocked at 18-21 hpf, in C at 24 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
fabp1a w30Tg standard conditions Protruding-mouth liver ISH
w30Tg heat shock Protruding-mouth liver ISH
fabp2 w30Tg standard conditions Protruding-mouth intestine ISH
w30Tg heat shock Protruding-mouth intestine ISH
GFP w30Tg heat shock 1-cell Not Detected whole organism WB
4-cell to Shield whole organism WB
prox1a w30Tg standard conditions Long-pec liver ISH
w30Tg heat shock Long-pec liver ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F59 IHC w30Tg standard conditions Long-pec somite
IHC w30Tg heat shock Long-pec somite
Phenotype Details
Fish Conditions Stage Phenotype
w30Tg heat shock Protruding-mouth intestine size, normal
Protruding-mouth liver aplastic, abnormal
Protruding-mouth liver decreased size, abnormal
Protruding-mouth liver development disrupted, abnormal
w30Tg heat shock Long-pec liver decreased size, abnormal
Long-pec liver development disrupted, abnormal
Long-pec liver position, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development