ZFIN Figure: Huang et al., 2008, Fig. S5

ZFIN ID: ZDB-FIG-081007-18

ZFIN ID: ZDB-FIG-081007-18

Huang et al., 2008 - Mypt1-mediated spatial positioning of Bmp2-producing cells is essential for liver organogenesis. Development (Cambridge, England) 135(19):3209-3218 Full text @ Development

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	fabp1a fabp2 GFP prox1a
Antibody:	Ab-F59
Fish:	w30Tg
Condition:	heat shock
Anatomical Terms:	intestine liver somite whole organism
Stage Range:	1-cell to Protruding-mouth

PHENOTYPE:

Fish:	w30Tg
Condition:	heat shock
Observed In:	intestine liver liver development
Stage Range:	Long-pec to Protruding-mouth

Fig. S5 Bmp signaling is essential for liver development in zebrafish. (A) Immunoblot analyses of dominant-negative BmpR1a-GFP expression using an anti-GFP antibody after heat-shock treatment. Total protein lysates were prepared from heat-shocked embryos at different time intervals as indicated. (B-D) WISH using the lfabp (B), prox1 (C) or ifabp (D) probes and F59 antibody (C) on heat-shocked embryos. Embryos in B and D were heat shocked at 18-21 hpf, in C at 24 hpf.

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
fabp1a	w30Tg	standard conditions	Protruding-mouth		liver	ISH
	w30Tg	heat shock	Protruding-mouth		liver	ISH
fabp2	w30Tg	standard conditions	Protruding-mouth		intestine	ISH
	w30Tg	heat shock	Protruding-mouth		intestine	ISH
GFP	w30Tg	heat shock	1-cell	Not Detected	whole organism	WB
			4-cell to Shield		whole organism	WB
prox1a	w30Tg	standard conditions	Long-pec		liver	ISH
	w30Tg	heat shock	Long-pec		liver	ISH

Antibody Labeling Details

Antibody	Assay	Fish	Conditions	Stage	Anatomy
Ab-F59	IHC	w30Tg	standard conditions	Long-pec	somite
	IHC	w30Tg	heat shock	Long-pec	somite

Phenotype Details

Fish	Conditions	Stage	Phenotype
w30Tg	heat shock	Protruding-mouth	intestine size, normal
		Protruding-mouth	liver aplastic, abnormal
		Protruding-mouth	liver decreased size, abnormal
		Protruding-mouth	liver development disrupted, abnormal
w30Tg	heat shock	Long-pec	liver decreased size, abnormal
		Long-pec	liver development disrupted, abnormal
		Long-pec	liver position, normal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development