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ZFIN ID: ZDB-FIG-080904-56
Kai et al., 2008 - Sphingosine-1-phosphate receptors regulate individual cell behaviours underlying the directed migration of prechordal plate progenitor cells during zebrafish gastrulation. Development (Cambridge, England)   135(18):3043-3051 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Bud
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Bud

Fig. 6 Edg1 acts antagonistically against Mil in the directed migration of prechordal plate progenitor cells. (A-F) In situ hybridisation of dlx, hgg1 and ntl of WT embryos injected with 6.2 ng edg1-MO alone (A,D) or co-injected with 4.3 ng mil-MO (B,E) at tailbud stage, and of slb embryos injected with 6.2 ng edg1-MO (C,F). (G-I) In situ hybridisation of dlx3 and hlx1 of WT embryos injected with edg1-MO alone (G) or co-injected with mil-MO (H) at tailbud stage, and of slb embryos injected with edg1-MO (I). (A-C,G-I) Dorso-animal views; (D-F) lateral views. edg1-MO injection reduced anterior migration of the anterior (A,D) and posterior (G) prechordal plate, compared with WT (see Fig. 1A,E,I), and enhanced the slb prechordal plate phenotype (C,F,I) (see Fig. 1C,G,K). Co-injection of edg1-MO and mil-MO cancelled out the effect of one another and often gave rise to an embryo reminiscent to WT (B,E,H).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb WT + MO1-s1pr1 standard conditions Bud prechordal plate ISH
WT + MO1-s1pr1 + MO1-s1pr2 standard conditions Bud prechordal plate ISH
wnt11f2tx226/tx226 + MO1-s1pr1 standard conditions Bud prechordal plate ISH
dlx3b WT + MO1-s1pr1 standard conditions Bud anterior neural plate ISH
WT + MO1-s1pr1 + MO1-s1pr2 standard conditions Bud anterior neural plate ISH
wnt11f2tx226/tx226 + MO1-s1pr1 standard conditions Bud anterior neural plate ISH
hlx1 WT + MO1-s1pr1 standard conditions Bud prechordal plate ISH
WT + MO1-s1pr1 + MO1-s1pr2 standard conditions Bud prechordal plate ISH
wnt11f2tx226/tx226 + MO1-s1pr1 standard conditions Bud prechordal plate ISH
tbxta WT + MO1-s1pr1 standard conditions Bud notochord ISH
WT + MO1-s1pr1 + MO1-s1pr2 standard conditions Bud notochord ISH
wnt11f2tx226/tx226 + MO1-s1pr1 standard conditions Bud notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-s1pr1 standard conditions Bud hypoblast mesodermal cell decreased speed, abnormal
Bud mesodermal cell migration decreased rate, abnormal
Bud prechordal plate decreased length, abnormal
WT + MO1-s1pr1 + MO1-s1pr2 standard conditions Bud hypoblast mesodermal cell speed, normal
Bud mesodermal cell migration rate, normal
wnt11f2tx226/tx226 + MO1-s1pr1 standard conditions Bud cell migration involved in gastrulation disrupted, abnormal
Bud convergent extension involved in gastrulation occurrence, normal
Bud notochord morphology, normal
Bud prechordal plate decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development