ZFIN ID: ZDB-FIG-080904-53
Kai et al., 2008 - Sphingosine-1-phosphate receptors regulate individual cell behaviours underlying the directed migration of prechordal plate progenitor cells during zebrafish gastrulation. Development (Cambridge, England)   135(18):3043-3051 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 50%-epiboly

Fig. 2 slb prechordal plate progenitors migrate individually with increased motility and reduced cohesion when Mil function is compromised. (A-I) Time-lapse analysis and tracking of cell movement in migrating prechordal plate progenitors of WT embryos (A-C), slb embryos (D-F) and slb embryos injected with 4.3 ng mil-MO (G-I). Five cells were randomly chosen from each embryo, highlighted in different colours and tracked by merging each starting point to 0 (C,F,I). Twenty-five cells in total were accumulated from five independent experiments, to analyze for speed, persistence and coherence (J). In slb embryos, cell motility was significantly reduced (D-F). In slb embryos injected with mil-MO, the cells showed increased motility, and the cluster of cells appeared to be less coherent (G-I); the cells occasionally popped out from the cluster and travelled individually (H,I, asterisk). Such cell behaviour was never observed in WT embryos. (J) Summary of analysis of parameters: speed, persistence and coherence. Statistically significant differences are indicated (#P<0.05, ##P<0.005).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
wnt11f2tx226/tx226 standard conditions 50%-epiboly cell migration involved in gastrulation disrupted, abnormal
50%-epiboly hypoblast mesodermal cell cellular motility, abnormal
50%-epiboly hypoblast mesodermal cell decreased speed, abnormal
50%-epiboly hypoblast mesodermal cell spatial pattern, abnormal
50%-epiboly mesodermal cell migration persistence, abnormal
wnt11f2tx226/tx226 + MO1-s1pr2 standard conditions 50%-epiboly cell migration involved in gastrulation disrupted, abnormal
50%-epiboly hypoblast mesodermal cell cellular motility, abnormal
50%-epiboly hypoblast mesodermal cell increased speed, abnormal
50%-epiboly hypoblast mesodermal cell spatial pattern, abnormal
50%-epiboly mesodermal cell migration persistence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development