ZFIN ID: ZDB-FIG-080904-52
Kai et al., 2008 - Sphingosine-1-phosphate receptors regulate individual cell behaviours underlying the directed migration of prechordal plate progenitor cells during zebrafish gastrulation. Development (Cambridge, England)   135(18):3043-3051 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Shield to Bud
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Shield to Bud

Fig. 1 Abrogation of Mil function facilitates anterior migration of the presumptive prechordal plate. In situ hybridisation (A-H,O,P) of dlx3 (anterior border of the neural plate), hgg1 (anterior prechordal plate) and ntl (notochord) at tailbud stage, (I-L) of dlx3 and hlx1 (posterior prechordal plate) at tailbud stage, and (M,N) of spry4 (presumptive mesoderm) at 60% epiboly. (A-D) Dorso-animal views, (E-H) lateral views, (I-L) dorso-animal views and (M-P) dorsal views of wild-type (WT) embryos (A,E,I,M), WT embryos injected with 4.3 ng mil-MO (B,F,J,N), slb embryos (C,G,K,O) and slb embryos injected with 4.3 ng mil-MO (D,H,L,P). In mil-MO-injected embryos, anterior migration of the presumptive prechordal plate was promoted (B,F), as compared to WT (A,E), with regards to relative positions of the prechordal plate in relation to the anterior border of the neural plate (A-D), and to angles between the prechordal plate and the tailbud, indicated by arrowheads (E-H). Injection of mil-MO rescued the reduced migration of the anterior prechordal plate in the slb embryo (C,D,G,H), but did not alter the wider notochord (O,P, indicated by asterisks). By contrast, the altered position of the posterior prechordal plate correlated with that of the anterior prechordal plate (K,L). (M,N) The enhancement of migration was not observed in the lateral mesoderm (brackets), but was specific to the prospective prechordal plate (arrow).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb WT standard conditions Bud prechordal plate ISH
WT + MO1-s1pr2 standard conditions Bud prechordal plate ISH
wnt11f2tx226/tx226 standard conditions Bud prechordal plate ISH
wnt11f2tx226/tx226 + MO1-s1pr2 standard conditions Bud prechordal plate ISH
dlx3b WT standard conditions Bud anterior neural plate ISH
WT + MO1-s1pr2 standard conditions Bud anterior neural plate ISH
wnt11f2tx226/tx226 standard conditions Bud anterior neural plate ISH
wnt11f2tx226/tx226 + MO1-s1pr2 standard conditions Bud anterior neural plate ISH
hlx1 WT standard conditions Bud prechordal plate ISH
WT + MO1-s1pr2 standard conditions Bud prechordal plate ISH
wnt11f2tx226/tx226 standard conditions Bud prechordal plate ISH
wnt11f2tx226/tx226 + MO1-s1pr2 standard conditions Bud prechordal plate ISH
spry4 WT standard conditions Shield presumptive mesoderm ISH
WT + MO1-s1pr2 standard conditions Shield presumptive mesoderm ISH
tbxta WT standard conditions Bud notochord ISH
WT + MO1-s1pr2 standard conditions Bud notochord ISH
wnt11f2tx226/tx226 standard conditions Bud notochord ISH
wnt11f2tx226/tx226 + MO1-s1pr2 standard conditions Bud notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-s1pr2 standard conditions Shield cell migration involved in gastrulation disrupted, abnormal
Bud cell migration involved in gastrulation disrupted, abnormal
Bud mesodermal cell migration increased rate, abnormal
Bud prechordal plate flat, abnormal
Bud prechordal plate increased length, abnormal
Bud prechordal plate increased width, abnormal
wnt11f2tx226/tx226 standard conditions Bud cell migration involved in gastrulation disrupted, abnormal
Bud convergent extension involved in gastrulation disrupted, abnormal
Bud mesodermal cell migration decreased rate, abnormal
Bud notochord decreased length, abnormal
Bud notochord increased thickness, abnormal
wnt11f2tx226/tx226 + MO1-s1pr2 standard conditions Bud cell migration involved in gastrulation occurrence, normal
Bud convergent extension involved in gastrulation disrupted, abnormal
Bud notochord decreased length, abnormal
Bud notochord increased thickness, abnormal
Bud prechordal plate increased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development