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ZFIN ID: ZDB-FIG-080826-12
Mizoguchi et al., 2008 - Sdf1/Cxcr4 signaling controls the dorsal migration of endodermal cells during zebrafish gastrulation. Development (Cambridge, England)   135(15):2521-2529 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 1-4 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 1-4 somites

Fig. 4 The C&E movements of mesoderm and ectoderm are not affected by Sdf1/Cxcr4a signaling during zebrafish gastrulation. (A-D) The position and shape of the prechordal plate (pcp), the anterior edge of the neural plate (np) and notochord (nt) at the three-somite stage. Embryos were stained for no tail (ntl; notochord marker), hatching gland gene 1 (hgg1; prechordal plate marker) and dlx3 (anterior edge of neuroectoderm) by in situ hybridization. Dorsal views, anterior to the top. No significant differences in the expression patterns of these three genes were found in either the cxcr4a or sdf1 knockdown embryos as compared with misMO1-injected embryos. White lines indicate the gap between the pcp and nt. (E-H) The body length of embryos at the three-somite stage, marked by the expression of myoD. Dorsal views, anterior to the top. The expression pattern of myoD in the cxcr4a or sdf1 knockdown embryos is indistinguishable from that in the misMO1-injected embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb WT control 1-4 somites prechordal plate ISH
WT + MO1-cxcl12b + MO3-cxcl12a standard conditions 1-4 somites prechordal plate ISH
WT + MO5-cxcr4a standard conditions 1-4 somites prechordal plate ISH
WT + MO6-cxcr4a standard conditions 1-4 somites prechordal plate ISH
dlx3b WT control 1-4 somites neural plate ISH
WT + MO1-cxcl12b + MO3-cxcl12a standard conditions 1-4 somites neural plate ISH
WT + MO5-cxcr4a standard conditions 1-4 somites neural plate ISH
WT + MO6-cxcr4a standard conditions 1-4 somites neural plate ISH
myod1 WT control 1-4 somites segmental plate ISH
1-4 somites somite ISH
WT + MO1-cxcl12b + MO3-cxcl12a standard conditions 1-4 somites segmental plate ISH
1-4 somites somite ISH
WT + MO5-cxcr4a standard conditions 1-4 somites segmental plate ISH
1-4 somites somite ISH
WT + MO6-cxcr4a standard conditions 1-4 somites segmental plate ISH
1-4 somites somite ISH
tbxta WT control 1-4 somites notochord ISH
WT + MO1-cxcl12b + MO3-cxcl12a standard conditions 1-4 somites notochord ISH
WT + MO5-cxcr4a standard conditions 1-4 somites notochord ISH
WT + MO6-cxcr4a standard conditions 1-4 somites notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cxcl12b + MO3-cxcl12a standard conditions 1-4 somites convergent extension involved in gastrulation process quality, normal
1-4 somites whole organism length, normal
WT + MO5-cxcr4a standard conditions 1-4 somites convergent extension involved in gastrulation process quality, normal
1-4 somites whole organism length, normal
WT + MO6-cxcr4a standard conditions 1-4 somites convergent extension involved in gastrulation process quality, normal
1-4 somites whole organism length, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development