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ZFIN ID: ZDB-FIG-080402-46
Lepage et al., 2008 - Characterization and comparative expression of zebrafish calpain system genes during early development. Developmental dynamics : an official publication of the American Association of Anatomists   237(3):819-829 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Shield to Long-pec

Fig. 5 Spatial expression patterns of zebrafish capn1, capn2, capns1, and cast genes during gastrulation, segmentation, and pharyngula stages. A-ZZZ: Representative whole-mount in situ hybridizations showing distribution of capn1a (A-D), capn1b (E-H), capn2a (I-L), capn2b (M-P), capns1a (Q-T), capns1b (U-X), and cast (Y-ZZZ) transcripts at shield stage (A,E,I,M,Q,U,Y), four to six somites (B,F,J,N,R,V,Z), 1 day (C,G,K,O,S,W,ZZ), and head regions of 2 day embryos (D,H,L,P,T,X,ZZZ). All embryos are shown in lateral view. Shield and 4-6 somite stage embryos are oriented with dorsal to the right. One day postfertilization (dpf) and 2 dpf embryos are oriented with anterior to the left. At 2 dpf, no staining was observed in structures posterior to the fin bud. Arrowheads indicate anterior mesendoderm (F,J,Z), polster (N,R), and proctodeum (O,S,W). Arrows indicate pronephros (S,W), pharynx (P,T,X,ZZZ), and hindbrain neuron clusters (H). The asterisk in W indicates the mid-hindbrain boundary region. hg, hatching gland.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
capn1a WT standard conditions Shield whole organism ISH
1-4 somites to 5-9 somites Not Detected whole organism ISH
Prim-5 brain ISH
Prim-5 Not Detected myotome ISH
Long-pec brain ISH
capn1b WT standard conditions Shield whole organism ISH
1-4 somites to 5-9 somites embryonic structure ISH
1-4 somites to 5-9 somites whole organism ISH
Prim-5 brain ISH
Prim-5 myotome ISH
Long-pec brain ISH
capn2a WT standard conditions Shield Not Detected whole organism ISH
1-4 somites to 5-9 somites embryonic structure ISH
Prim-5 brain ISH
Long-pec brain ISH
Long-pec pectoral fin ISH
Long-pec retina ISH
capn2b WT standard conditions Shield shield ISH
1-4 somites to 5-9 somites polster ISH
Prim-5 brain ISH
Prim-5 Not Detected hatching gland ISH
Prim-5 proctodeum ISH
Long-pec brain ISH
Long-pec pectoral fin ISH
Long-pec pharynx ISH
capns1a WT standard conditions Shield whole organism ISH
1-4 somites to 5-9 somites polster ISH
Prim-5 brain ISH
Prim-5 epidermal superficial stratum ISH
Prim-5 hatching gland ISH
Prim-5 pronephros ISH
Long-pec pectoral fin ISH
Long-pec pharynx ISH
capns1b WT standard conditions Shield whole organism ISH
1-4 somites to 5-9 somites whole organism ISH
Prim-5 brain ISH
Prim-5 epidermal superficial stratum ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 proctodeum ISH
Prim-5 pronephros ISH
Long-pec brain ISH
Long-pec pectoral fin ISH
Long-pec pharynx ISH
cast WT standard conditions Shield whole organism ISH
1-4 somites to 5-9 somites embryonic structure ISH
1-4 somites to 5-9 somites periderm ISH
Prim-5 brain ISH
Prim-5 proctodeum ISH
Long-pec brain ISH
Long-pec pectoral fin ISH
Long-pec pharynx ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.