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ZIRC
ZFIN ID: ZDB-FIG-080402-43
Lepage et al., 2008 - Characterization and comparative expression of zebrafish calpain system genes during early development. Developmental dynamics : an official publication of the American Association of Anatomists   237(3):819-829 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: 64-cell to Prim-5

Fig. 2 Temporal expression pattern and exon organization of four calpastatin splice variants. Semiquantitative reverse transcriptase (RT) -polymerase chain reaction using cast F1 and R1 primers was used to determine the relative abundance of four cast transcripts at 64-cell, sphere, shield, bud, and 1 day postfertilization (dpf). One representative no template control (no RT) is shown and β-actin was used as a loading control. Schematic representation of exon organization is shown for each of the four transcripts. cast F1 and R1 primers amplify exons 1-9 and F2 and R2 amplify exons 11-20 as indicated. cast F2 and R2 amplify a single product (see Fig. 3).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cast WT standard conditions 64-cell to Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.