ZFIN ID: ZDB-FIG-071228-29
Holtzinger et al., 2007 - Gata5 and Gata6 are functionally redundant in zebrafish for specification of cardiomyocytes. Developmental Biology   312(2):613-622 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5

Fig. 6 The defect in gata5+6 morphants is specific to cardiac mesoderm. Shown in each panel is a typical representative embryo following processing by whole mount in situ hybridization. Embryos were either wild-type (A, C, E, G) or gata5(ssG5)+6 double morphants (B, D, F, H). (A, B) The cardiomyocyte progenitor marker nkx2.5 at the 12-somite stage shows the lack of cardiac progenitors in gata5+6 morphants. (C, D) The pronephric marker pax2.1 shows that the intermediate mesoderm was not affected in morphants. (E, F) The axial mesoderm marker no tail shows that gata5+6 morphants develop a normal notochord. (G, H) The lateral plate mesoderm, marked by the lmo2 probe, is also not altered in gata5+6 morphants. (A and B) views are dorsal, with anterior to the top. (C, D, G, and H) embryos were flat mounted, views are dorsal, with anterior to the left. (E and F) Views are from the left side, with anterior to the left. These panels represent patterns seen in A: 39/39; B: 38/38; C: 10/10; D: 23/23; E: 26/26; F: 45/45; G: 28/28; H: 42/42.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
lmo2 WT standard conditions Prim-5 lateral plate mesoderm ISH
WT + MO1-gata5 + MO1-gata6 standard conditions Prim-5 lateral plate mesoderm ISH
nkx2.5 WT standard conditions 10-13 somites heart primordium ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 10-13 somites Not Detected heart primordium ISH
pax2a WT standard conditions 10-13 somites intermediate mesoderm ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 10-13 somites intermediate mesoderm ISH
tbxta WT standard conditions 20-25 somites notochord ISH
20-25 somites trunk ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 20-25 somites notochord ISH
20-25 somites trunk ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 312(2), Holtzinger, A., and Evans, T., Gata5 and Gata6 are functionally redundant in zebrafish for specification of cardiomyocytes, 613-622, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.