hcrtr Is Expressed in Neither the Posterior Diencephalic Dopaminergic Cells nor the Locus Coeruleus of Adult Zebrafish Brain. The topmost panels display lateral views of zebrafish adult brains with transversal plane corresponding to sections presented below. Results were all obtained using ISH. (A–C) hcrtr mRNA is expressed in the periventricular gray zone of optic tectum (pgz), periventricular nucleus of the posterior tuberculum (tpp), posterior tuberal nucleus (ptn), and ventral zone of periventricular hypothalamus (hv) of the anterior diencephalon (A). In the posterior diencephalon (B), hcrtr mRNA is mainly detected in the periventricular gray zone of optic tectum, medial preglomerular nucleus (pgm), and hypophysis (hp). hcrtr has a sparse expression in the anterior rhombencephalon (C), including the griseum centrale (gc) near the locus coeruleus. (D–F) th, dat, and dbh expression patterns in sections similar to those shown in (A–C), respectively. th is expressed in posterior tuberal nucleus and ventral zone of periventricular hypothalamus and in the torus longitudinalis (tl) (D). dat expression is restricted to a few posterior tuberal nucleus cells in the posterior diencephalon (E). dbh is expressed in the locus coeruleus (lc) (F). (G–I) Double ISH on equivalent sections showing colocalization of hcrtr (blue) with th (red) in the anterior catecholaminergic/dopaminergic cells of the diencephalon (G), absence of colocalization of hcrtr (blue) with dat (red) in the posterior catecholaminergic/dopaminergic cells of the diencephalon (H), and absence of colocalization of hcrtr (blue) with dbh (red) in the catecholaminergic/noradrenergic locus coeruleus (I).
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