hcrtr Does Not Colocalize with the Cholinergic System in the Telencephalon and Rhombencephalon. The topmost panels display lateral views of zebrafish adult brains with transversal planes corresponding to sections presented below. Results were all obtained using ISH with chat and hcrtr. (A) Cholinergic neurons were not detected in the adult zebrafish telencephalon using chat or vacht (data not shown). In this area, hcrtr is strongly expressed in the commissural nucleus of the ventral telencephalic area (cv) and the anterior part of the parvocellular preoptic nucleus (ppa). (B and C) Major clusters of cholinergic neurons are present in the periventricular gray zone (pgz) ([B], panel 1), the nucleus of the medial longitudinal fascicle (nmlf) ([B], panel 2), the perilemniscal nucleus (pl) ([B], panel 3), the dorsal zone of periventricular hypothalamus (hd) ([B], panel 4), and the nucleus lateralis valvulae (nlv) (C). Coexpression with hcrtr was mostly observed in the periventricular gray zone ([B], panel 1) and in the dorsal zone of periventricular hypothalamus ([B], panel 4). (D–G) A careful survey of the rhombencephalon failed to reveal coexpression of hcrtr with chat-positive neurons, including in the peri-locus coeruleus area.
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