PUBLICATION
Characterization of Sleep in Zebrafish and Insomnia in Hypocretin Receptor Mutants
- Authors
- Yokogawa, T., Marin, W., Faraco, J., Pézeron, G., Appelbaum, L., Zhang, J., Rosa, F., Mourrain, P., and Mignot, E.
- ID
- ZDB-PUB-071023-9
- Date
- 2007
- Source
- PLoS Biology 5(10): 2379-2397 (Journal)
- Registered Authors
- Mourrain, Philippe, Pézeron, Guillaume, Rosa, Frederic
- Keywords
- Sleep, Zebrafish, Mammals, Functional electrical stimulation, Hypothalamus, Sleep disorders, Sleep deprivation, Narcolepsy
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Arousal/physiology
- Behavior, Animal/physiology
- Biogenic Monoamines/metabolism
- Brain/anatomy & histology
- Brain/metabolism
- Homeostasis
- Humans
- Intracellular Signaling Peptides and Proteins/metabolism
- Light
- Molecular Sequence Data
- Neuropeptides/metabolism
- Orexin Receptors
- Orexins
- Receptors, G-Protein-Coupled/genetics
- Receptors, G-Protein-Coupled/metabolism*
- Receptors, Neuropeptide/genetics
- Receptors, Neuropeptide/metabolism*
- Sequence Alignment
- Sleep/physiology*
- Sleep Deprivation
- Sleep Initiation and Maintenance Disorders/metabolism*
- Zebrafish/anatomy & histology
- Zebrafish/embryology
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 17941721 Full text @ PLoS Biol.
Citation
Yokogawa, T., Marin, W., Faraco, J., Pézeron, G., Appelbaum, L., Zhang, J., Rosa, F., Mourrain, P., and Mignot, E. (2007) Characterization of Sleep in Zebrafish and Insomnia in Hypocretin Receptor Mutants. PLoS Biology. 5(10):2379-2397.
Abstract
Sleep is a fundamental biological process conserved across the animal kingdom. The study of how sleep regulatory networks are conserved is needed to better understand sleep across evolution. We present a detailed description of a sleep state in adult zebrafish characterized by reversible periods of immobility, increased arousal threshold, and place preference. Rest deprivation using gentle electrical stimulation is followed by a sleep rebound, indicating homeostatic regulation. In contrast to mammals and similarly to birds, light suppresses sleep in zebrafish, with no evidence for a sleep rebound. We also identify a null mutation in the sole receptor for the wake-promoting neuropeptide hypocretin (orexin) in zebrafish. Fish lacking this receptor demonstrate short and fragmented sleep in the dark, in striking contrast to the excessive sleepiness and cataplexy of narcolepsy in mammals. Consistent with this observation, we find that the hypocretin receptor does not colocalize with known major wake-promoting monoaminergic and cholinergic cell groups in the zebrafish. Instead, it colocalizes with large populations of GABAergic neurons, including a subpopulation of Adra2a-positive GABAergic cells in the anterior hypothalamic area, neurons that could assume a sleep modulatory role. Our study validates the use of zebrafish for the study of sleep and indicates molecular diversity in sleep regulatory networks across vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping