Fig. 2

Brain-Specific Loss of Asymmetry and Excessive Diencephalic Nodal Activation in six3b/six7-Deficient Embryos (A–F) Left-sided expression in control (A, C, and E) and bilateral expression in six3b/six7-deficient embryos (B, D, and F) of lft1 (A and B), pitx2 (C and D), and cyc (E and F). In the LPM, expression remains left-sided (arrows in insets [A–D]). (E–H) The diencephalic domain of Nodal expression and activity is expanded in six3b/six7-deficient embryos (compare [F] to [E] and [H] to [G]). lft1 expression extends outside the flh-positive pineal anlage (H). (I and J) oep expression continues to coincide with the pineal anlage in six3b/six7-deficient embryos (J). Control embryos in all panels are six3b^vu87/+ or six3b^vu87/vu87 and present normal phenotypes. R, right; L, left. (A–D) Dorsoposterior views; insets show smaller magnification of the same embryo in each panel. (E–J) Dorsal views, anterior is up. All embryos are 21–24s stage. Scale bars, 100 μm.