Fig. 1

Combined Loss of Six3b/Six7 Function Results in Lack of Eye Tissue and Abnormal Brain Laterality (A) Schematic presentation of normal Six3b and the predicted truncated Six3b protein, encoded by the six3b^vu87 allele. (B and C) Live control embryo (B) and a six3b/six7-deficient embryo (B) demonstrating lack of eye tissue. (D and E) Comparable brain patterning in 24 hpf control (D) and six3b/six7-deficient (E) embryos. Forebrain (fb), midbrain-hindbrain boundary (mhb), and hindbrain (hb) are labeled with emx3, pax2a, and egr2b, respectively. The optic stalk (os, arrowheads, pax2a) is reduced in the six3b/six7-deficient embryo. (F and G) Pineal (arrow) and parapineal localization (arrowheads) in control (F) and six3b/six7-deficient (G) embryos. (H and I) Habenular nuclei labeled with lov, in control (H) and six3b/six7-deficient (I) embryos. Control embryos are six3b^vu87/+ or six3b^vu87/vu87 and present normal phenotypes. R, right; L, left. Anterior is to the left in (B)–(E) and up in (F)–(I). (B)–(E) are lateral views, and (F)–(I) and insets in (B) and (C) are dorsal views. Scale bars, 200 μm.