FIGURE

Fig. 8

ID

ZDB-FIG-070730-3

Publication

Robu et al., 2007 - p53 activation by knockdown technologies

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Fig. 8

Quantitative RT-PCR of p21/WAF/CIP in Various Morphants. p21 RNA levels were significantly increased in MO-injected embryos that showed neural death (Smo MO, Wnt5 MO1, and Mdm2 MO) as compared to the uninjected control (UI). Interestingly, p21 levels were not significantly altered by Wnt5 MO2 (which does not induce neural death). Very importantly, p21 levels were significantly reduced in the respective MO-injected embryos upon p53 co-knockdown (Mdm2 MO p < 0.01, Wnt5 MO2 p < 0.01, Smo MO p < 0.002; t-test p values for unpaired sets), strongly suggesting that p21 is transcriptionally upregulated by p53 in the morphants with neural death. Error bars indicate standard deviation.

Expression Data

Gene:	cdkn1a
Fish:	WT WT + MO1-mdm2 WT + MO1-mdm2 + MO4-tp53 WT + MO2-wnt5b WT + MO2-wnt5b + MO4-tp53 WT + MO3-wnt5b WT + MO4-smo WT + MO4-smo + MO4-tp53
Knockdown Reagents:	MO1-mdm2 MO2-wnt5b MO3-wnt5b MO4-smo MO4-tp53
Anatomical Term:	whole organism
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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