Expression Patterns of SP2035, SP2054, and SP2063. In situ hybridization for SP2054, SP2035, and SP2063 showed that all three transcripts were localized in anterior structures prior to chondrogenesis (1 dpf). Later in development, SP2054 and SP2035 transcripts became localized in pharyngeal arch structures during cartilage formation (2 dpf, see Figure S2; and 3 dpf), while SP2063 mRNA was expressed in brain structures.Please see Figure S2 for a higher resolution version that also includes a 2 dpf time point. AB, anterior brain; CNS, central nervous system; MHB, midbrain/hindbrain boundary; OS, optic stalk; PA, pharyngeal arch; V, vasculature.
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