Expression of genes that mark `early' versus differentiated hematopoietic cells in zebrafish nz171 mutants, and partial rescue of differentiated cells by microinjection of runx1 mRNA. (A,B) Expression of runx1 and gata1 is altered in nz171 mutants. Flat preparations of 10-somite embryos, anterior to the left. Expression of runx1, gata1 and scl in the PLM (A), and pu.1 in the ALM and PLM (B) of wild-type siblings and nz171 mutants. gata1 expression in nz171 mutants is noticeably reduced compared with wild type. (C,D) Expression of genes that mark differentiated hematopoietic cells is reduced or absent in nz171 mutants. Whole-mount embryos, anterior to the left. (C) Reduced expression of hbbe3 (hemoglobin beta embryonic3) in nz171 mutant embryos (lower panels) compared with wild type (upper panels). (D) Loss of lyz (lysozyme) expression in nz171 is rescued by microinjection of runx1 mRNA. Upper left, whole-mount 26 h.p.f. embryo stained for lyz expression. The box indicates the region shown at higher magnification in the other panels. Upper panels, wild-type expression of lyz and cebpg in 26 h.p.f. embryos, as indicated. Lower center and right, similar views of 26 h.p.f. nz171 homozygotes, showing no expression of lyz and cebpg. Lower left panel, rescued expression of lyz in 26 h.p.f. embryos indicated by white arrowheads (21/23 mutant embryos rescued).
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