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ZFIN ID: ZDB-FIG-070619-20
Stetsyuk et al., 2007 - Calsenilin is required for endocrine pancreas development in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists   236(6):1517-1525 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Prim-5 to Prim-25
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Prim-25

Fig. 4 Pancreatic endocrine development is affected in calsenilin knockdown embryos. A: In vitro efficacy of morpholino antisense oligonucleotides directed against calsenilin. B: Whole-mount in situ hybridization on embryos injected with MO-calsenilin, MO3-calsenilin or with 5-misMO-calsenilin control antisense oligonucleotides. Zebrafish embryos at 24 hours postfertilization (hpf) or 40 hpf were hybridized with a digoxigenin-labeled antisense insulin probe. Quantification of insulin-positive cells either in 5-misMO-calsenilin, MO-calsenilin, or MO3-calsenilin morphants at stage 24 hpf. In knockdown embryos, the number of insulin-expressing cells was strongly decreased. ***P < 0.0001 C: Whole-mount in situ hybridization with digoxigenin-labeled antisense sst2, pdx1, isl1, and pax6.2 in MO-calsenilin and control 5-misMO-calsenilin embryos at 24 hpf. Note that in MO-calsenilin morphants, pancreatic endocrine cells were dispersed and did not associate to form an islet. Quantification of sst2-, isl1-, and Pax6.2-positive cells in wild-type or MO-calsenilin morphants at stage 24 hpf. *P < 0.01; ***P < 0.0001

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ins WT control Prim-5 pancreatic bud ISH
Prim-25 pancreatic bud ISH
WT standard conditions Prim-5 pancreatic bud ISH
WT + MO1-kcnip3b standard conditions Prim-5 pancreatic bud ISH
Prim-25 pancreatic bud ISH
WT + MO2-kcnip3b standard conditions Prim-5 pancreatic bud ISH
isl1a WT control Prim-5 pancreatic bud ISH
WT + MO1-kcnip3b standard conditions Prim-5 pancreatic bud ISH
pax6b WT control Prim-5 pancreatic bud ISH
WT + MO1-kcnip3b standard conditions Prim-5 pancreatic bud ISH
pdx1 WT control Prim-5 pancreatic bud ISH
WT + MO1-kcnip3b standard conditions Prim-5 pancreatic bud ISH
sst2 WT control Prim-5 pancreatic bud ISH
WT standard conditions Prim-5 pancreatic bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-kcnip3b standard conditions Prim-5 endocrine pancreas disorganized, abnormal
Prim-25 endocrine pancreas disorganized, abnormal
WT + MO2-kcnip3b standard conditions Prim-5 endocrine pancreas disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.