Expression of calsenilin in the zebrafish pancreas. A: Search for new genes expressed in the pancreas: zebrafish at 24 hours postfertilization (hpf) were cohybridized with digoxigenin-labeled antisense probes (in blue) corresponding to the gene to be tested and fluorescein-labeled antisense insulin probe (in red). Calsenilin that was coexpressed with insulin was kept for further analysis. B: Expression of calsenilin during zebrafish development. Whole-mount in situ hybridization was performed with digoxigenin-labeled antisense calsenilin and insulin probes at different stages of development (21-somites, 24 hpf, 29 hpf, 32 hpf, 39 hpf). Arrows point to insulin-positive regions.
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