Calsenilin is required for endocrine pancreas development in zebrafish
- Stetsyuk, V., Peers, B., Mavropoulos, A., Verbruggen, V., Thisse, B., Thisse, C., Motte, P., Duvillie, B., and Scharfmann, R.
- Developmental dynamics : an official publication of the American Association of Anatomists 236(6): 1517-1525 (Journal)
- Registered Authors
- Mavropoulos, Anastasia, Peers, Bernard, Thisse, Bernard, Thisse, Christine, Verbruggen, Vincianne
- pancreas, calsenilin, development, zebrafish
- MeSH Terms
- Animals, Genetically Modified
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Endocrine System/embryology*
- Endocrine System/metabolism*
- Gene Expression Regulation, Developmental
- Kv Channel-Interacting Proteins/genetics
- Kv Channel-Interacting Proteins/metabolism*
- Pancreatic Hormones/metabolism
- Receptors, Notch/metabolism
- Signal Transduction
- 17450605 Full text @ Dev. Dyn.
Stetsyuk, V., Peers, B., Mavropoulos, A., Verbruggen, V., Thisse, B., Thisse, C., Motte, P., Duvillie, B., and Scharfmann, R. (2007) Calsenilin is required for endocrine pancreas development in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 236(6):1517-1525.
Calsenilin/DREAM/Kchip3 is a neuronal calcium-binding protein. It is a multifunctional protein, mainly expressed in neural tissues and implicated in regulation of presenilin processing, repression of transcription, and modulation of A-type potassium channels. Here, we performed a search for new genes expressed during pancreatic development and have studied the spatiotemporal expression pattern and possible role of calsenilin in pancreatic development in zebrafish. We detected calsenilin transcripts in the pancreas from 21 somites to 39 hours postfertilization stages. Using double in situ hybridization, we found that the calsenilin gene was expressed in pancreatic endocrine cells. Loss-of-function experiments with anti-calsenilin morpholinos demonstrated that injected morphants have a significant decrease in the number of pancreatic endocrine cells. Furthermore, the knockdown of calsenilin leads to perturbation in islet morphogenesis, suggesting that calsenilin is required for early islet cell migration. Taken together, our results show that zebrafish calsenilin is involved in endocrine cell differentiation and morphogenesis within the pancreas.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes