PUBLICATION

Calsenilin is required for endocrine pancreas development in zebrafish

Authors
Stetsyuk, V., Peers, B., Mavropoulos, A., Verbruggen, V., Thisse, B., Thisse, C., Motte, P., Duvillie, B., and Scharfmann, R.
ID
ZDB-PUB-070504-13
Date
2007
Source
Developmental dynamics : an official publication of the American Association of Anatomists   236(6): 1517-1525 (Journal)
Registered Authors
Mavropoulos, Anastasia, Peers, Bernard, Thisse, Bernard, Thisse, Christine, Verbruggen, Vincianne
Keywords
pancreas, calsenilin, development, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Endocrine System/embryology*
  • Endocrine System/metabolism*
  • Gene Expression Regulation, Developmental
  • Kv Channel-Interacting Proteins/genetics
  • Kv Channel-Interacting Proteins/metabolism*
  • Mutation/genetics
  • Pancreas/embryology*
  • Pancreas/metabolism*
  • Pancreatic Hormones/metabolism
  • Receptors, Notch/metabolism
  • Signal Transduction
  • Tretinoin/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed
17450605 Full text @ Dev. Dyn.
Abstract
Calsenilin/DREAM/Kchip3 is a neuronal calcium-binding protein. It is a multifunctional protein, mainly expressed in neural tissues and implicated in regulation of presenilin processing, repression of transcription, and modulation of A-type potassium channels. Here, we performed a search for new genes expressed during pancreatic development and have studied the spatiotemporal expression pattern and possible role of calsenilin in pancreatic development in zebrafish. We detected calsenilin transcripts in the pancreas from 21 somites to 39 hours postfertilization stages. Using double in situ hybridization, we found that the calsenilin gene was expressed in pancreatic endocrine cells. Loss-of-function experiments with anti-calsenilin morpholinos demonstrated that injected morphants have a significant decrease in the number of pancreatic endocrine cells. Furthermore, the knockdown of calsenilin leads to perturbation in islet morphogenesis, suggesting that calsenilin is required for early islet cell migration. Taken together, our results show that zebrafish calsenilin is involved in endocrine cell differentiation and morphogenesis within the pancreas.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes