PUBLICATION

Calsenilin is required for endocrine pancreas development in zebrafish

Authors

Stetsyuk, V., Peers, B., Mavropoulos, A., Verbruggen, V., Thisse, B., Thisse, C., Motte, P., Duvillie, B., and Scharfmann, R.

ID

ZDB-PUB-070504-13

Date

2007

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 236(6): 1517-1525 (Journal)

Registered Authors

Mavropoulos, Anastasia, Peers, Bernard, Thisse, Bernard, Thisse, Christine, Verbruggen, Vincianne

Keywords

pancreas, calsenilin, development, zebrafish

MeSH Terms

Animals
Pancreas/embryology*
Pancreas/metabolism*
Signal Transduction
Pancreatic Hormones/metabolism
Receptors, Notch/metabolism
Mutation/genetics
Gene Expression Regulation, Developmental
Zebrafish/embryology*
Zebrafish/genetics
Zebrafish/metabolism*
Endocrine System/embryology*
Endocrine System/metabolism*
Animals, Genetically Modified
Kv Channel-Interacting Proteins/genetics
Kv Channel-Interacting Proteins/metabolism*
Embryo, Nonmammalian/embryology
Embryo, Nonmammalian/metabolism
Tretinoin/metabolism

(all 19)

PubMed

17450605 Full text @ Dev. Dyn.

Abstract

Calsenilin/DREAM/Kchip3 is a neuronal calcium-binding protein. It is a multifunctional protein, mainly expressed in neural tissues and implicated in regulation of presenilin processing, repression of transcription, and modulation of A-type potassium channels. Here, we performed a search for new genes expressed during pancreatic development and have studied the spatiotemporal expression pattern and possible role of calsenilin in pancreatic development in zebrafish. We detected calsenilin transcripts in the pancreas from 21 somites to 39 hours postfertilization stages. Using double in situ hybridization, we found that the calsenilin gene was expressed in pancreatic endocrine cells. Loss-of-function experiments with anti-calsenilin morpholinos demonstrated that injected morphants have a significant decrease in the number of pancreatic endocrine cells. Furthermore, the knockdown of calsenilin leads to perturbation in islet morphogenesis, suggesting that calsenilin is required for early islet cell migration. Taken together, our results show that zebrafish calsenilin is involved in endocrine cell differentiation and morphogenesis within the pancreas.

Genes / Markers

Figures

Show all Figures

Expression

Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
WT	standard conditions	Prim-15		pancreatic bud	ISH	Fig. 2
aldh1a2^i26/i26	standard conditions	Prim-5	Not Detected	pancreatic bud	ISH	Fig. 3
mib1^ta52b/ta52b	standard conditions	Prim-5		pancreatic bud	ISH	Fig. 3
WT	standard conditions	20-25 somites to Prim-25		pancreas primordium	ISH	Fig. 1
WT	control	Prim-5		pancreatic bud	ISH	Fig. 4
WT	standard conditions	Prim-15		pancreatic bud	ISH	Fig. 2
WT	standard conditions	Prim-5		pancreatic bud	ISH	Fig. 4
WT	control	Prim-25		pancreatic bud	ISH	Fig. 4
WT	standard conditions	20-25 somites to Prim-25		pancreatic bud	ISH	Fig. 1
WT	standard conditions	Prim-5		pancreatic bud	ISH	Fig. 2

1 - 10 of 36

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-kcnip3b	standard conditions	Prim-5	endocrine pancreas disorganized, abnormal	Fig. 4
WT + MO1-kcnip3b	standard conditions	Prim-25	endocrine pancreas disorganized, abnormal	Fig. 4
WT + MO2-kcnip3b	standard conditions	Prim-5	endocrine pancreas disorganized, abnormal	Fig. 4

1 - 3 of 3

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
i26		Point Mutation	aldh1a2
ta52b		Point Mutation	mib1

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
kcnip3b	MO1-kcnip3b	MRPHLNO
kcnip3b	MO2-kcnip3b	MRPHLNO

Fish

Fish
aldh1a2^i26/i26
mib1^ta52b/ta52b
WT
WT + MO1-kcnip3b
WT + MO2-kcnip3b

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Stetsyuk et al., 2007 ZDB-PUB-070504-13 PMID:17450605

Calsenilin is required for endocrine pancreas development in zebrafish

Stetsyuk et al., 2007

ZDB-PUB-070504-13
PMID:17450605